镉诱导雏鸡肝脏的损伤作用  

Cadmium-induced liver damage in chicks

作　　者：顾成霞 王艳 张宇 唐中齐 叶瑞旗 汪梦杰 刘畅[1,2,3] 曹迷霞 李磊 GU Chengxia;WANG Yan;ZHANG Yu;TANG Zhongqi;YE Ruiqi;WANG Mengjie;LIU Chang;CAO Mixia;LI Lei(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China;Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,Fengyang 233100,China;Key Laboratory of Quality&Safety Control for Pork,Ministry of Agriculture and Rural Affairs,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]动物营养调控与健康安徽省重点实验室,安徽凤阳233100 [3]农业农村部猪肉质量安全控制重点实验室,安徽凤阳233100

出　　处：《安徽科技学院学报》2024年第1期18-23,共6页Journal of Anhui Science and Technology University

基　　金：国家自然科学基金(31802242);安徽省自然科学基金(2008085QC146);安徽省高校自然科学研究重点项目(2022AH051640,2023AH051885);安徽科技学院人才引进项目(ZRC2014447);安徽省重点研究与开发计划项目(202004a06020050);国家级大学生创新创业训练计划项目(202110879063);安徽省大学生创新创业训练计划项目(S202110879178)。

摘　　要：目的:旨在观察镉对雏鸡肝脏的损伤作用,为预防、治疗和诊断镉致雏鸡肝损伤提供依据。方法:200只1日龄健康爱拨益加雏鸡随机分为空白对照组、CdCl_(2)处理组(低、中、高剂量),每组5个重复,每个重复10只。CdCl_(2)处理组分别灌服35、70、140 mg/kg·BW的CdCl2溶液。雏鸡染镉24 h后,心脏采血处死动物并分离肝组织,利用苏木精-伊红染色观察肝组织病理变化,测定血清中转氨酶活性与肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(Interleukin-1β,IL-1β)含量,比色法测定肝组织中氧化应激指标。结果:35、70 mg/kg CdCl_(2)处理可引起雏鸡血清中转氨酶活性显著提高(P<0.01),雏鸡肝细胞出现空泡变性、炎性浸润和细胞坏死等病理改变;GSH含量、SOD活性显著降低(P<0.01),MDA含量急剧升高(P<0.01),血清中炎性细胞因子IL-1β、IL-6与TNF-α含量显著升高(P<0.01)。结论:CdCl_(2)可导致雏鸡肝组织出现氧化应激,并伴随炎症反应。Objective:To observe the effects of cadmium on liver injury of chicks,and to provide evidence for prevention,treatment and diagnosis of cadmium-induced liver injury of chicks.Methods:A total of 2001-day-old healthy Aidiega chicks were randomly divided into control group and CdCl_(2) treatment groups(low,medium and high dose)with 5 replicates per group and 10 chicks per replicate.CdCl_(2) treatment groups were given 35,70,140 mg/kg·BW of CdCl_(2) solution,respectively.After 24 h of cadmium treatment,heart blood was collected and the animals were killed and the liver tissues were separated.Hematoxylin-eosin staining(H&E)was used to observe the pathological changes of liver tissues.The activity of transaminase and the contents of tumor necrosis factor-α(T NF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in serum were determined,and the indexes of oxidative stress in liver tissue were determined by colorimetry.Results:35,70 mg/kg CdCl_(2) could significantly increase the activity of aminotransferase in serum of chicks(P<0.01).The pathological changes such as vacuolar degeneration,inflammatory infiltration and cell death were observed in chicken liver cells.GSH content and SOD activity were significantly decreased(P<0.01),MDA content was significantly increased(P<0.01),and serum inflammatory cytokines IL-1β,IL-6 and TNF-αcontents were significantly increased(P<0.01).Conclusion:CdCl_(2) can induce oxidative stress in liver tissue of chicks,accompanied by inflammation.

关 键 词：镉 雏鸡 肝损伤 氧化应激 

分 类 号：S859.87[农业科学—临床兽医学]