细菌介导重金属转化过程及金属组学研究方法  

Metallomic Methods in Bacteria-mediated Transformation of Heavy Metals

作　　者：唐寅寅 刘丽红[1] 何滨[1,2,3] 胡立刚 TANG Yinyin;LIU Lihong;HE Bin;HU Ligang(State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China;School of Environment,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang 310024,China)

机构地区：[1]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [2]中国科学院大学,北京100049 [3]国科大杭州高等研究院环境学院,杭州310024

出　　处：《中国无机分析化学》2024年第1期26-34,共9页Chinese Journal of Inorganic Analytical Chemistry

基　　金：国家自然科学基金资助项目(22076200,22276201)。

摘　　要：重金属污染依然是我国严重的环境问题之一。在重金属胁迫下,微生物可通过复杂的过程,对重金属进行转化,降低重金属的毒性,其中转化涉及的分子机制广受关注。目前,在上述机制相关研究中,对转化过程中重金属的衡量多局限于总量的测定,而对其赋存形态的研究不足,导致难以取得有效进展。对细菌介导重金属的转化过程及金属在其中的赋存形态进行综述,探讨了金属组学研究方法在其中的应用,重点针对重金属的颗粒态与蛋白质结合态进行分析、表征和鉴定,为微生物介导重金属的转化研究提供新视角。Heavy metal pollution is still one of the most serious environmental problems in China.Microorganisms can reduce the toxicity of heavy metals in the environment through complex process of transformation under stress of heavy metal,and the mechanism of transformation has received wide attention.In current mechanistic studies,the investigation of the transformation process is limited to the determination of total metals,but the speciation study of heavy metals is inadequate,which has led to difficulties in achieving breakthroughs.The bacterial-mediated transformation processes of heavy metals and the applied metallomic approaches has been reviewed,especially for analysis,characterization and identification of the particles forms and protein-binding species of heavy metals,which provides a new perspective for the study of microbial-mediated heavy metal transformation.

关 键 词：重金属污染 金属组学 转化 细菌介导 

分 类 号：O657.63[理学—分析化学]