非优质胚胎囊胚激光辅助孵化对新生儿出生体重的影响  

Effect of laser-assisted hatching on birth weight in blastocyst culture of low-grade cleavage embryos

作　　者：李施施[1] 朱忠林 舒崇医 徐维海 LI Shishi;ZHU Zhonglin;SHU Chongyi;XU Weihai(Department of Reproductive Endocrinology,Zhejiang Provincial People's Hospital,Hangzhou,Zhejiang 310014,China;Anhui University of Science and Technology,Huainan,Anhui 232001,China;Reproductive Medicine Center,The First Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou,Zhejiang 310003,China)

机构地区：[1]浙江省人民医院生殖内分泌科,浙江杭州310014 [2]安徽理工大学,安徽淮南232001 [3]浙江大学医学院附属第一医院生殖医学中心,浙江杭州310003

出　　处：《预防医学》2024年第2期169-172,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省自然科学基金项目(LGF21H040008)。

摘　　要：目的 探讨非优质胚胎进行囊胚培养时应用激光辅助孵化(LAH)技术对子代围产期结局的影响,为提升非优质胚胎利用效率、改善胚胎发育质量提供依据。方法 选择在浙江省人民医院接受体外受精/单精子卵胞浆内注射后移植解冻囊胚获得单活胎出生的子代369例为研究对象,根据囊胚培养第4天是否实施LAH分为常规组(51例)和LAH组(318例),收集胎龄、出生体重、出生缺陷及母亲围产期相关资料;两组采用倾向得分匹配后,分析早产、出生体重和出生缺陷情况。结果 匹配后,常规组和LAH组各49例,母亲年龄、体质指数、不孕类型和囊胚龄等基本资料差异均无统计学意义(P>0.05),匹配有效。LAH组子代出生体重为(3 261.08±432.24) g,低于常规组的(3 464.18±444.46) g (P<0.05)。结论 非优质胚胎进行囊胚培养时应用LAH可能降低子代的出生体重。Objective To examine the impact of laser-assisted hatching(LAH)technique on perinatal outcomes in blas⁃tocyst culture of low-grade cleavage embryos,so as to provide insights into improving the utilization of low-grade cleav⁃age embryos and embryo quality.Methods A total of 369 single live births after transfer of thawed blastocysts follow⁃ing in vitro fertilization or intracytoplasmic sperm injection at Zhejiang Provincial People's Hospital were selected as sub⁃jects,and they were divided into 51 conventional culture blastocysts and 318 LAH blastocysts based on whether LAH was performed on day 4 of blastocyst culture.Gestational age,birth weight,birth defects and maternal perinatal informa⁃tion were collected,and the prevalence of premature birth,birth weight and birth defects were analyzed after propensity score matching(PSM).Results After PSM,98 matched cases were included in the study.There were no statistically significant differences in maternal age,body mass index,type of infertility and blastocyst age(P>0.05),indicating effec⁃tive matching.The birth weight of offspring in the LAH group was lower than that in the conventional culture group[(3261.08±432.24)g vs.(3464.18±444.46)g;P<0.05].Conclusion The birth weight of offspring can be reduced by using LAH during blastocyst culture of low-grade cleavage embryos.

关 键 词：不孕症 囊胚 激光辅助孵化 出生体重 巨大儿 

分 类 号：R714.8[医药卫生—妇产科学]