槲皮素对大鼠心肌梗死后左心室重塑的改善机制研究  

Study on the ameliorating mechanism of quercetin on left ventricular remodeling after myocardial infarction in rats

作　　者：罗建 朱彬[1] 刘应才 范新荣 魏燕 LUO Jian;ZHU Bin;LIU Yingcai;FAN Xinrong;WEI Yan(Department of Cardiology,Luzhou Hospital of Traditional Chinese Medicine,Luzhou,Sichuan 646000,China;Department of Cardiology,The Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China;Institute of Cardiovascular Research,Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]泸州市中医医院心血管内科,四川泸州646000 [2]西南医科大学附属医院心血管内科,四川泸州646000 [3]西南医科大学心血管医学研究所,四川泸州646000

出　　处：《重庆医学》2024年第3期328-332,338,共6页Chongqing medicine

基　　金：四川省泸州市科技计划项目(2021-JYJ-77)。

摘　　要：目的探讨槲皮素(Que)对大鼠心肌梗死(MI)后心室重塑的保护作用及对炎症细胞因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的影响。方法将30只SD雄性大鼠随机分为假手术组(Sham组)、MI组和Que组,每组各10只。MI组采用结扎左前降支构建模型,Sham组只穿针不结扎,Que组在MI后予以槲皮素100 mg·kg^(-1)·d^(-1)喂养。喂养28 d后,予以心脏彩色多普勒超声评估心脏结构和功能,马松(Masson)染色测定心肌纤维化程度,ELISA法观察大鼠血液循环中TNF-α和IL-6水平,免疫组织化学染色(IHC)检测心脏组织中TNF-α和IL-6表达水平。结果MI组较Sham组左心室收缩末期容量(LVESv)及左心室舒张末期容量(LVEDv)增大,左心室射血分数(LVEF)及左心室短轴收缩率(LVFS)下降,左心室心肌纤维化程度升高,循环中及心脏组织中TNF-α和IL-6表达水平升高,差异有统计学意义(P<0.05);Que组较MI组LVESv缩小,LVEF及LVFS上升,左心室心肌纤维化程度下降,循环中及心脏组织中TNF-α和IL-6表达水平降低,差异有统计学意义(P<0.05)。结论Que可明显改善大鼠MI后左心室重塑,其机制可能与其减少炎症细胞因子TNF-α和IL-6的释放相关。Objective To investigate the protective effect of quercetin(Que)on ventricular remodeling in rats with myocardial infarction(MI)and its effect on inflammatory cytokines tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).Methods A total of 30 SD male rats were randomly divided into the Sham operation group(Sham group),MI group and Que group,10 rats in each group.The MI group was modeled by ligation of the left anterior descending branch,the Sham group was only punctured without ligation,and the Que group was fed with quercetin 100 mg·kg^(-1)·d^(-1)after MI.After 28 days of feeding,cardiac color Doppler ultrasound was used to evaluate cardiac structure and function.Masson staining was used to determine the degree of myocardial fibrosis.ELISA was used to observe the levels of TNF-αand IL-6 in blood circulation of rats.Immunohistochemical staining(IHC)was used to detect the expression levels of TNF-αand IL-6 in heart tissue.Results Compared with the Sham group,the left ventricular end-systolic volume(LVESv)and left ventricular end-diastolic volume(LVEDv)were increased,the left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)were decreased,the degree of left ventricular myocardial fibrosis was increased,and the expression levels of TNF-αand IL-6 in the circulation and heart tissues were increased in the MI group,and the differences were statistically significant(P<0.05).Compared with the MI group,LVESvwas decreased,LVEF and LVFS were increased,the degree of left ventricular myocardial fibrosis was decreased,and the expression levels of TNF-αand IL-6 in circulation and heart tissue were decreased in the Que group,and the differences were statistically significant(P<0.05).Conclusion Que can significantly improve left ventricular remodeling after MI in rats,and its mechanism may be related to the reduction of the release of inflammatory cytokines TNF-αand IL-6.

关 键 词：槲皮素 心肌梗死 心室重塑 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R54[医药卫生—心血管疾病]