一种适用于检测水相中肼的荧光探针研究  

Research of a Fluorescent Probe Detecting for Hydrazine in Water Phase

作　　者：王磊[1] 李雪 吴佳佳 刘佳蕊 陈奕辛 甘静 张文静[2] WANG Lei;LI Xue;WU Jia-jia;LIU Jia-rui;CHEN Yi-xin;GAN Jing;ZHANG Wen-jing(School of Science,Xuchang University,Xuchang 461000,China;Green Catalysis Center and College of Chemistry,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]许昌学院数理学院,河南许昌461000 [2]郑州大学化学学院(绿色催化中心),河南郑州450001

出　　处：《化学试剂》2024年第2期88-94,共7页Chemical Reagents

基　　金：许昌学院科研专项项目(2023ZX008);河南省高等学校大学生创新创业训练计划项目(202310480008)。

摘　　要：肼是一种重要的化工原料,具有广泛的用途。肼的毒性对环境和生物体具有重要影响,然而能够检测水相中肼的荧光探针非常有限。设计合成了一种新的反应型荧光探针,通过核磁共振和高分辨质谱对中间产物和探针分子进行了结构表征。研究发现探针能够特异性的检测水相中的肼,具有较高的灵敏度和强抗干扰能力。通过荧光光谱和理论计算相结合,探究了探针的检测机理和发光性质。研究发现探针分子与肼接触,发生肼解反应,导致荧光团恢复激发态分子内质子转移(ESIPT)发光,从而实现对肼的特异性检测。Hydrazine serves as a chemical raw material with diverse applications,but its toxicity significantly impacts the environment and living organisms.However,fluorescent probes capable of detecting hydrazine in water phase are very limited.To address this issue,a novel reactive fluorescence probe was conceptualized and synthesized.The intermediate and probe structures were characterized by NMR and high-resolution mass spectrometry.It was found that the probe exhibits specific detection of hydrazine in water phase,with high sensitivity and strong anti-interference ability.By combining fluorescence spectra and theoretical calculations,the detection mechanism and luminescence properties of the probe were investigated.It was found that upon contact with hydrazine,the probe undergoes hydrazolysis,leading to the recovery of luminescence through the Excited-State Intramolecular Proton Transfer(ESIPT)process,thereby achieving specific detection of hydrazine.

关 键 词：荧光探针 肼 水相 ESIPT 理论计算 

分 类 号：O657.3[理学—分析化学]