白腐菌与厌氧污泥共培养强化秸秆湿式厌氧发酵  

Enhanced wet anaerobic digestion of straw by co-culture of white-rot fungusand anaerobic sludge

作　　者：袁文典 于麒麟 张耀斌[1] YUAN Wendian;YU Qilin;ZHANG Yaobin(Key Laboratory of Industrial Ecology and Environmental Engineering,Ministry of Education,School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China)

机构地区：[1]大连理工大学环境学院工业生态与环境工程教育部重点实验室,辽宁大连116024

出　　处：《能源环境保护》2024年第1期76-84,共9页Energy Environmental Protection

基　　金：国家重点研发计划课题资助项目(2023YFD1701401);国家自然科学基金资助项目(22276024)。

摘　　要：秸秆是广泛存在的可再生生物质资源之一,但由于木质素复合体的难降解性,当前湿式厌氧发酵对秸秆利用程度普遍较低。本研究通过将一种具有高效降解木质素能力的白腐菌——黄孢原毛平革菌与厌氧污泥在微氧条件下共培养,并通过秸秆的湿式发酵,探究共培养对秸秆悬液产甲烷的影响。结果表明,在0.6 mg/L溶解氧条件下,与非共培养组相比,共培养组的底物甲烷产率提高了45%(达到75.76 mL/g VS),TCOD去除率提高了37%。电化学分析结果显示,共培养能有效提高污泥电容(提高了182.9%)。黄孢原毛平革菌的引入使秸秆更易被利用,并且秸秆代谢产生的电活性物质加强了腐殖化过程及污泥胞外电子传递,从而增加了甲烷的产量。本研究实现了对未经预处理的秸秆高效厌氧发酵,为秸秆资源化提供借鉴。Straw is one of the widely available renewable biomass resources.However,due to the difficult degradability of the lignin structure,wet anaerobic fermentation is generally not highly utilized for straw at present.In this study,Phanerochaete chrysosporium,a white-rot fungus,was co-cultivated with anaerobic sludge under microaerobic conditions to investigate its effect on methane production from straw in wet anaerobic fermentation.The results showed that the substrate methane yield(75.76 mL/g VS)and TCOD removal in the co-culture group increased by 45%and 37%,respectively,compared to the non-co-culture group at 0.6 mg/L dissolved oxygen.Electrochemical analysis revealed that co-culture effectively increased the sludge capacitance by 182.9%.The introduction of Phanerochaete chrysosporium made the straw easier to digest and produced electroactive substances,which intensified the humification process and extracellular electron transfer,and consequently increased methane production.Effective utilization of straw without pretreatment was achieved in this study,providing a reference for further straw resource recovery.

关 键 词：秸秆 湿式厌氧消化 黄孢原毛平革菌 微氧 腐殖化 

分 类 号：X505[环境科学与工程—环境工程] X172