初沉出水与消化液优化配比培养小球藻的研究  

Research on the cultivation of Chlorella by optimizing the ratio ofprimary settling effluent and digestive solution

作　　者：孙慧娣 杨廷浩 韩习习 刘长青[1] 赵方超[1] SUN Huidi;YANG Tinghao;HAN Xixi;LIU Changqing;ZHAO Fangchao(School of Environmental and Municipal Engineering,Qingdao University of Technology,Qingdao 266520,China;Shanghai Municipal Engineering Design and Research Institute(Group)Co.,Ltd.,Shanghai 200092,China)

机构地区：[1]青岛理工大学环境与市政工程学院,山东青岛266520 [2]上海市政工程设计研究总院(集团)有限公司,上海200092

出　　处：《能源环境保护》2024年第1期143-150,共8页Energy Environmental Protection

基　　金：山东省自然科学基金博士基金资助项目(ZR2019BEE028)。

摘　　要：本研究旨在基于前期筛选和驯化获取抗污能力较强的蛋白核小球藻,将其接种到市政污水处理厂的初沉池出水与污泥消化液不同比例(1∶0、3∶1、1∶1、1∶3和0∶1)混合的污水中,研究污水中小球藻生长速率、油脂产率以及污染物去除率。结果表明,在比例为3∶1的污水中培养小球藻可以获得最高的藻浓度(0.704 g/L)。利用比例为1∶1的污水培养小球藻,可以获得最佳的总体产油效果,产油率达到36.29%。小球藻对5种比例污水的NH_(4)^(+)-N、TN和PO_4^(3-)-P均表现出较好的去除效果,但对化学需氧量(COD)的去除效果不佳,且在培养过程中NO_(3)^(-)-N浓度一直处于较低水平。小球藻对比例为1∶0、3∶1和1∶1污水中的TP有较好的去除效果,而对比例为1∶3和0∶1污水中的TP去除效果不佳。综合考虑,初沉池出水与污泥消化液比例为3∶1时培养小球藻是较为合适的选择。This study was conducted using Chlorella pyrenoidosa based on previous screening and domestication.The Chlorella was inoculated into sewage mixed with the effluent from the primary sedimentation tank and sludge digestion liquid in different proportions(1∶0,3∶1,1∶1,1∶3,and 0∶1),observing the growth rate and oil yield of Chlorella pyrenoidosa,as well as the pollutant removal rate in the sewage.The results indicated that the highest algal concentration of 0.704 g/L was achieved when Chlorella was cultured in sewage with a ratio of 3∶1.Furthermore,the best oil production effect and maximum oil production rate of 36.29%were observed when Chlorella was cultured in sewage with a ratio of 1∶1.Chlorella demonstrated effective removal of NH_(4)^(+)-N,TN,and PO_4^(3-)-P in sewage across all ratios,but had a poor removal effect on COD.Additionally,the concentration of NO_(3)^(-)-N remained consistently low throughout the culture process.Chlorella exhibited better removal of TP in sewage with ratios of 1∶0,3∶1,and 1∶1,while the ratios of 1∶3 and 0∶1 showed less effective removal.Overall,the most favorable Chlorella culture was achieved in sewage with a ratio of 3∶1.

关 键 词：污水资源化 小球藻 生长速率 油脂产率 污染物去除率 

分 类 号：X703[环境科学与工程—环境工程]