青海田鼠源鼠突线虫的分子鉴定与进化分析  

MOLECULAR IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF MASTOPHORUS MURIS ISOLATES FROM MICROTUS FUSCUS

作　　者：师正合 付永[2] 张学勇[2] 曹杰 孟茹 别文海 顾玉姣 靳燕婴 王宏宾 SHI Zheng-he;FU Yong;ZHANG Xue-yong;CAO Jie;MENG Ru;BIE Wen-hai;GU Yu-jiao;JIN Yan-ying;WANG Hong-bin(School of Clinical Medicine,Qinghai University,Xining 810016,Qinghai,China;Academy of Animal and Veterinary Sciences,Qinghai University,Xining 810016,Qinghai,China;Xining Animal Disease Control Center,Xining 810016,Qinghai,China;Gangcha County Animal Husbandry and Veterinary Station,Gangcha 812300,Qinghai,China)

机构地区：[1]青海大学临床医学院,西宁810016 [2]青海大学畜牧兽医科学院,西宁810016 [3]西宁市动物疫病预防控制中心,西宁810016 [4]刚察县畜牧兽医站,青海刚察812300

出　　处：《寄生虫与医学昆虫学报》2023年第4期197-201,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金项目(32160840);青海省应用基础研究项目(2021-ZJ-724)。

摘　　要：为鉴定青海田鼠源线虫种类,通过PCR方法扩增该线虫样本的线粒体COI基因序列,对扩增产物进行测序和序列比对,并进行系统发育分析。结果表明,获得的青海田鼠源线虫分离株与鼠突线虫Mastophorus muris一致,同源性可达98.6%;种系发育进化树表明,该分离株与GenBank中已公布的其他线虫类所属分支相距较远,而与M.muris(MK8674)处于同一分支并且亲缘关系最近,属于胃寄生线虫的鼠突线虫。本研究首次从分子水平上鉴定出青海田鼠源鼠突线虫,可进一步为青藏高原鼠突线虫流行病学调查提供科学依据。This study aims to identify the species of nematodes originating from Microtus fuscus.Mitochondrial COI gene sequences of the nematode samples were amplified using PCR,and the resulting amplification products were sequenced.The obtained sequences were compared and subjected to phylogenetic analysis,revealing that the nematode species isolated from M.fuscus is identical to Mastophorus muris,exhibiting a high homology of 98.6%with M.muris(MK8674).The germline evolutionary tree demonstrated that the isolate is distantly related to other nematodes documented in GenBank,but shares a branch with M.muris(MK8674),representing its closest relative.This nematode belongs to the rat-parasitic nematodes of the stomach.This study marks the first molecular level identification of M.muris from Qinghai voles,providing a scientific basis for further epidemiological investigations of M.muris on the Qinghai-Tibet Plateau.

关 键 词：青海田鼠 鼠突线虫 分子鉴定 种系发育 

分 类 号：R383.1[医药卫生—医学寄生虫学]