不同浓度萝芙木提取物对良性前列腺增生大鼠细胞增殖的影响  

Rauwolfia extract inhibits the proliferation of prostate cells in rats with benign prostatic hyperplasia

作　　者：刘彪 方天 梁磊 胡文娟 董敏 陈莉 吴伟 恽时锋 LIU Biao;FANG Tian;LIANG Lei;HU Wen-juan;DONG Min;CHEN Li;WU Wei;YUN Shi-feng(Department of Laboratory Animals,General Hospital of Eastern Theater Command,Nanjing,Jiangsu 210002 China)

机构地区：[1]解放军东部战区总医院医疗保障中心实验动物室,江苏南京210002

出　　处：《中华男科学杂志》2023年第3期195-201,共7页National Journal of Andrology

基　　金：全军实验动物专项课题面上项目(SYDW[2018]06号);全军实验动物专项课题青年项目(SYDW[2017]15号)。

摘　　要：目的:探讨不同浓度的萝芙木提取物(RE)对BPH模型大鼠前列腺组织中细胞增殖的影响。方法:SD大鼠去势后,皮下注射丙酸睾酮[5mg/(kg·d)]构建BPH模型,将BPH大鼠随机分为BPH模型组、非那雄胺组、RE低浓度组、RE中浓度组、RE高浓度组,未造模的SD大鼠为正常对照组。非那雄胺组大鼠按5 mg/kg体重灌胃非那雄胺溶液,RE低、中、高浓度组分别按照浓度为5、10、20 mg/kg体重灌胃RE溶液,对照组和BPH模型组灌胃等剂量生理盐水,1次/d,连续28 d。实验结束处死大鼠,取前列腺组织,称量前列腺湿重,容积法检测前列腺体积,计数前列腺指数,检测血清中睾酮和双氢睾酮含量,HE染色观察前列腺组织结构变化,结合图像分析系统,半定量检测前列腺组织腺体数目、腺体管腔面积,并采用免疫组化检测增殖细胞核抗原PCNA和α-SMA阳性表达率。结果:与对照组相比较,BPH模型组的前列腺湿重、前列腺体积和前列腺指数均显著升高[(1.455±0.52)g vs(0.923±0.15)g,(1.687±0.31)ml vs(1.035±0.29)ml,(0.37±0.15)%vs(0.23±0.04)%,P<0.05];腺体平均数目显著减少[(12.56±2.58)vs(20.35±3.83),P<0.01];上皮厚度增加约2倍[(39.76±5.20)μm vs(19.52±1.52)μm,P<0.01];腺体内管腔面积减少了50%左右[(5.96±0.34)×10^(3)μm^(2)vs(12.3±1.21)×10^(3)μm^(2),P<0.01]。并且前列腺间质和血管扩张充血水肿,细胞增殖速率明显增高。RE和非那雄胺处理后均可以逆转上述结果。不同浓度RE处理后前列腺湿重显著降低、前列腺体积明显减小及前列腺重量指数显著降低,其中高浓度组效果最显著[(0.862±0.31)g vs(1.455±0.52)g,(0.952±0.28)ml vs(1.687±0.31)ml,(0.22±0.07)%vs(0.37±0.15)%,P<0.05]。不同浓度RE处理后前列腺组织病理学改变改善明显,腺体数目、上皮厚度和腺体内面积显著回归,RE高浓度组效果最为明显[(18.36±1.25)vs(12.56±2.58),(19.04±3.89)μm vs(39.76±5.20)μm,(10.25±0.98)×10^(3)μm^(2)vs(5.96±0.34)×10^Objective:To investigate the effects of different concentrations of Rauwolfia extract(RE)on the proliferation of prostate cells in the rat model of benign prostatic hyperplasia(BPH).Methods:We randomly divided 48 male SD rats into six groups of an equal number,BPH model control,finasteride,low-concentration RE,medium-concentration RE,high-concentration RE and normal control,and established a BPH model in the former five groups by subcutaneous injection of testosterone propionate following castration.We treated the rats of the finasteride and RE groups intragastrically with finasteride solution at 5 mg/kg and RE at 5,10 and 20 mg/kg respectively,and those of the model control and normal control groups with an equal dose of normal saline,all once a day for 28 consecutive days.Then,we killed all the animals,collected their prostate tissue,obtained the wet weight and volume of the prostate,the prostate index and the contents of serum T and dihydrotestosterone(DHT),observed the morphological changes of the prostate tissue by HE staining,counted the glands in the prostate tissue,measured the intraglandular area,and determined the expressions of PCNA andα-SMA by immunohistochemistry.Results:Compared with the rats of the normal control group,the BPH model controls showed significantly increased wet weight([0.923±0.15]vs[1.455±0.52]g,P<0.05),volume([1.035±0.29]vs[1.687±0.31]ml,P<0.05)and index of the prostate([0.23±0.04]%vs[0.37±0.15]%,P<0.05),dilation,hyperemia and edema of the prostatic stroma and vessels,and proliferation rate of the prostatic cells,but remarkably decreased number of glands([20.35±3.83]vs[12.56±2.58],P<0.05),epithelial thickness([39.76±5.20]vs[19.52±1.52]μm,P<0.05)and intraglandular area([12.3±1.21]vs[5.96±0.34]×10^(3)μm^(2),P<0.05).In comparison with the BPH model controls,the animals treated with RE,especially in the high-concentration RE group,exhibited marked decreases in the weight([1.455±0.52]vs[0.862±0.31]g,P<0.05),volume([1.687±0.31]vs[0.952±0.28]ml,P<0.05)and index of the p

关 键 词：萝芙木提取物 良性前列腺增生 增殖细胞核抗原 α平滑肌肌动蛋白抗体 大鼠 

分 类 号：R697.32[医药卫生—泌尿科学]