WIF-1在COPD合并骨骼肌萎缩大鼠中的表达及其与血清炎症因子、Atrogin-1/MuRF-1蛋白水平的关系  

Expression of WIF-1 and its relation to serum inflammatory factors,Atrogin-1/MuRF-1 protein levels in COPD combined with skeletal muscle atrophy rats

作　　者：王晓薇 许艳岚 WANG Xiao-wei;XU Yan-lan(Department of Geriatrics,Qingpu Branch of Zhongshan Hospital,Fudan University,Shanghai 201700,China)

机构地区：[1]复旦大学附属中山医院青浦分院老年科,上海201700

出　　处：《临床和实验医学杂志》2024年第1期1-5,共5页Journal of Clinical and Experimental Medicine

基　　金：上海市卫健委科研辅助项目(编号:201840069)。

摘　　要：目的探讨WIF-1在慢性阻塞性肺疾病(COPD)合并骨骼肌萎缩大鼠中的表达及其与血清炎症因子、Atrogin-1/MuRF-1蛋白水平的关系。方法将30只SD大鼠按照随机数字表法分为COPD组(仅建立COPD成功的大鼠,n=11)、COPD合并骨骼肌萎缩组(COPD合并营养不良骨骼肌萎缩大鼠,n=9)和对照组(未建立动物模型的健康大鼠,n=10),分别给予相应的处理。采用酶联免疫吸附试验法检测3组大鼠的血清白细胞介素(IL)-6、IL-8及肿瘤坏死因子(TNF)-α水平;苏木精-伊红染色观察肺组织及骨骼肌腓肠肌组织形态;免疫组织化学检测骨骼肌组织中WIF-1细胞阳性表达率;采用蛋白质印迹法检测骨骼肌组织中Atrogin-1、MuRF-1蛋白表达;Pearson法分析WIF-1与Atrogin-1、MuRF-1相关性。结果与对照组相比,COPD组大鼠IL-6、IL-8及TNF-α水平均明显升高,差异均有统计学意义(P<0.05);与COPD组相比,COPD合并骨骼肌萎缩组大鼠IL-6、IL-8及TNF-α水平均明显升高,差异均有统计学意义(P<0.05)。与对照组相比,COPD组大鼠WIF-1细胞阳性表达率较高,但差异无统计学意义(P>0.05);与COPD组相比,COPD合并骨骼肌萎缩组大鼠WIF-1细胞阳性表达率明显升高,差异有统计学意义(P<0.05)。与对照组相比,COPD组大鼠Atrogin-1、MuRF-1蛋白水平增加,但差异无统计学意义(P>0.05);与COPD组相比,COPD合并骨骼肌萎缩组大鼠Atrogin-1、MuRF-1蛋白水平均明显增加,差异均有统计学意义(P<0.05)。WIF-1与Atrogin-1呈正相关(r=0.760,P<0.001),WIF-1与MuRF-1无相关性(r=0.149,P=0.433)。结论WIF-1基因在COPD合并骨骼肌萎缩大鼠中阳性表达显著升高,具有促炎症因子反应的作用,同时可影响肌肉萎缩相关蛋白而促进疾病进展。Objective To investigate the expression of WIF-1 and its relationship with serum inflammatory factors,Atrogin-1/MuRF-1 protein levels in chronic obstructive pulmonary disease(COPD)combined with skeletal muscle atrophy rats.Methods Thirty SD rats were divided into the COPD group(rats with successful COPD model establishment,n=11),the COPD combined with skeletal muscle atrophy group(rats with COPD combined with malnutrition-induced skeletal muscle atrophy,n=9)and the control group(healthy rats without animal model establishment,n=10)according to the random number table method,and treated accordingly.The levels of serum interleukin(IL)-6,IL-8,and tumor necrosis factor(TNF)-αin the three groups of rats were detected by enzyme-linked immunosorbent assay;lung tissue and gastrocnemius muscle tissue morphology was observed by Hematoxylin-Eosin staining;the cell-positive expression rate of WIF-1 in skeletal muscle tissue was detected by immunohistochemistry;the protein expression of Atrogin-1 and MuRF-1 in skeletal muscle tissue was detected by Western blotting;the correlation between WIF-1 and Atrogin-1,MuRF-1 was analyzed by Pearson's method.Results Compared with the control group,the levels of IL-6,IL-8,and TNF-αin the COPD group were significantly increased,the differences were statistically significant(P<0.05);compared with the COPD group,the levels of IL-6,IL-8,and TNF-αin the COPD combined with skeletal muscle atrophy group were significantly increased,the differences were statistically significant(P<0.05).Compared with the control group,the cell-positive expression rate of WIF-1 in the COPD group rats was higher,but the difference was not statistically significant(P>0.05);compared with the COPD group,the cell-positive expression rate of WIF-1 in the COPD combined with skeletal muscle atrophy group was significantly increased,the differences were statistically significant(P<0.05).Compared with the control group,the protein levels of Atrogin-1 and MuRF-1 in the COPD group were increased,but the differences were n

关 键 词：大鼠 慢性阻塞性肺疾病 骨骼肌萎缩 WIF-1 ATROGIN-1 MuRF-1 炎症因子 

分 类 号：R563.9[医药卫生—呼吸系统] R-332[医药卫生—内科学]