鲜食玉米自交系苗期耐水淹种质筛选及生理特性分析  

Screening and Physiological Analysis of Fresh-eating Corn with Waterlogging Tolerance at Seedling Stage

作　　者：张金池 关媛[2,3,4] 秦涛 党冬冬 于典司[2,3,4] 顾炜 王慧[2,3,4] 郑洪建[2,3,4] 白光红[1] Zhang Jinchi;Guan Yuan;Qin Tao;Dang Dongdong;Yu Diansi;Gu Wei;Wang Hui;Zheng Hongjian;Bai Guanghong(College of Agriculture,Xinjiang Agricultural University,Urumqi,830052;Crop Breeding&Cultivation Research Institution,CYMMIT-China Specialty Maize Research Center,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Shanghai Engineering Research Center of SpecialtyMaize,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai Academy of Agricultural Sciences,Shanghai,201403)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]上海市农业科学院作物育种栽培研究所,CIMMYT-中国特用玉米研究中心,上海201403 [3]上海市农业科学院,上海特用玉米工程技术研究中心,上海201403 [4]上海市农业科学院,上海市农业遗传育种重点实验室,上海201403

出　　处：《分子植物育种》2024年第3期823-831,共9页Molecular Plant Breeding

基　　金：上海市科技兴农项目(沪农科创字[2019]第1-1号);上海市科技计划项目(20392000400)共同资助。

摘　　要：在鲜食玉米苗期发生涝害能够造成较大幅度减产,通过遗传改良提高鲜食玉米苗期的抗涝性是应对水淹胁迫的有效途径。本研究以241份鲜食玉米自交系为研究材料,以水淹胁迫下苗期自交系株高、植株干重的变化率为评价指标,筛选耐水淹和水淹敏感鲜食玉米自交系各20份。此外,在水淹胁迫下,对筛选品种苗期叶片的光合指标、胁迫相关生理指标及耐水淹相关标记基因ZmP4H4、Sicyp51、ZmbRI的变化进行分析。结果表明:对于光合指标、水淹敏感自交系与耐水淹自交系存在显著差异。耐水淹自交系处理与对照的丙二醛含量无显著差异(P=0.3603),但过氧化氢含量存在显著差异(P=0.0456),脯氨酸的含量存在极显著差异(P=0.016)。水淹敏感自交系处理与对照的丙二醛(P=0.0479)和脯氨酸含量(P=0.036)存在显著差异,过氧化氢(P=6.63E-06)含量存在极显著差异。耐水淹自交系处理与对照之间耐涝相关基因表达量变化较明显,而水淹敏感自交系处理与对照之间耐涝相关基因表达量变化不显著。研究结果对于玉米耐水淹育种具有重要意义。The occurrence of waterlogging in fresh maize seedling stage can cause a relatively large yield reduction,and improving the waterlogging resistance of fresh maize seedling stage through genetic improvement is an effective way to cope with flooding stress.In this study,241 fresh maize inbred lines were used as research materials,and the change rate of plant height and plant dry weight of inbred lines at seedling stage under flooding stress was used as the evaluation index to screen the waterlogging-resistant and flooding-sensitive fresh maize inbreds 20 copies each.In addition,under flooding stress,the changes of photosynthetic indexes,stress-related physiological indexes and flood-tolerance-related marker genes ZmP4H4,Sicyp51 and ZmbRI were analyzed in the leaves of the screened varieties at seedlin g stage.The results showed that there were significant differences between the flood-sensitive inbred lines and the flood-resistant inbred lines for photosynthetic indicators.There was no significant difference in the content of malondialdehyde between the flood-resistant inbred line treatment and the control(P=0.3603),but there was a significant difference in the content of hydrogen peroxide(P=0.0456),and there was an extremely significant difference in the content of proline(P=0.016).There were significant differences in the content of malondialdehyde(P=0.0479)and proline(P=0.036),and the content of hydrogen peroxide(P=6.63E-06)between the flood-sensitive inbred lines and the control.The expression of waterlogging tolerance-related genes changed significantly between the flood-tolerant inbred line treatment and the control,while the waterlogging tolerance-related gene expression did not change significantly between the flooding-sensitive inbred line treatment and the control.The results of this study have important implications for maize breeding for flood tolerance.

关 键 词：鲜食玉米 苗期 耐水淹胁迫 光合指标 生理指标 耐涝标记基因 

分 类 号：S513[农业科学—作物学]