八棱海棠胚再生体系的建立  

Establishment of Embryo Regeneration System of Malus robusta Rehd.

作　　者：雷富臣 封明军 豆静 王新建 Lei Fuchen;Feng Mingjun;Dou Jing;Wang Xinjian(National and Local Joint Engineering Laboratory of High-Efficiency and High-quality Cultivation and Deep Processing Technology of Southern Xinjiang Characteristic Fruit Trees,College of Plant Science,Tarim University,Alar,843300)

机构地区：[1]塔里木大学植物科学学院,南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,阿拉尔843300

出　　处：《分子植物育种》2024年第3期849-858,共10页Molecular Plant Breeding

基　　金：新疆生产建设兵团重大科技项目(2016jb03-1)资助。

摘　　要：为建立高效的八棱海棠胚再生体系,本研究检测和分析了八棱海棠种子消毒处理、胚诱导、组培苗增殖、生根以及叶片愈伤组织诱导的影响因素。结果表明:最优的消毒处理剂为,剥除种皮前:75%乙醇/30s+10%NaClO_(2)/10 min,剥除种皮后:10%NaClO_(2)/1 min,可将种子的污染率降至0,且发芽率最高为93%;组培苗最优增殖培养基配比为MS+2.0 mg/L 6-BA+0.1 mg/L NAA,增殖系数为4.11,苗高为2.57 cm;组培苗最适生根培养基配方为1/2MS+0.7 mg/LIBA+0.5 mg/LNAA,组培苗经培养后其生根率达到100%,平均根长为7.97 cm,主根数为8.33,根系表面积为54.71 cm^(2),根尖数为572.27,平均株高3.42 cm;叶片愈伤组织诱导最适培养基配比为MS+0.1 mg/L TDZ+1.0 mg/L NAA和MS+2.5 mg/L 2,4-D,诱导率为94.70%和91.33%。本研究结果为八棱海棠的快速繁殖提供参考。In order to establish an efficient embryo regeneration system of Malus robusta Rehd.,this study tested and analyzed the factors affecting the sterilization of Malus robusta Rehd.seeds,embryo induction,tissue culture seedling proliferation,rooting,and leaf callus induction.The results show that the optimal disinfectant is 75% ethanol/30 s+10%NaClO_(2)/10 min before seed coat removal,and 10%NaClO_(2)/1 min after seed coat removal,which can reduce the contamination rate of seeds 0,and the highest germination rate is 93%;the optimal growth medium ratio for tissue culture seedlings is MS+2.0 mg/L 6-BA+0.1 mg/L NAA,the growth coefficient is 4.11,and the seedling height is 2.57 cm;the optimumrooting medium formula for tissue culture seedlings is 1/2MS+0.7 mg/L IBA+0.5 mg/L NAA,the rooting rate of tissue cultured seedlings reaches 100% after culture,the average root length is 7.97 cm,the number of main roots is 8.33,and the root surface area is 54.71 cm^(2),the number of root tips is 572.27,and the average plant height is 3.42 cm;the optimal medium ratio for leaf callus induction is MS+0.1 mg/L TDZ+1.0 mg/L NAA and MS+2.5 mg/L 2,4-D,the induction rate was 94.70% and 91.33%.This experiment provides a reference for the rapid propagation of Malus robusta Rehd..

关 键 词：八棱海棠 胚 组织培养 快速繁殖 

分 类 号：S685.99[农业科学—观赏园艺]