白癜风小鼠模型抑郁样行为的观察  

Analysis of depression-like behavioral performances of mouse models of vitiligo

作　　者：孙玮蔚 陈健儒 李舒丽 高天文 李春英 Sun Weiwei;Chen Jianru;Li Shuli;Gao Tianwen;Li Chunying(Department of Dermatology,Xijing Hospital,Air Force Medical University,Xi′an 710032,China)

机构地区：[1]空军军医大学西京皮肤医院,西安710032

出　　处：《中华皮肤科杂志》2024年第2期147-154,共8页Chinese Journal of Dermatology

基　　金：国家自然科学基金(81972928)。

摘　　要：目的观察分析白癜风模型小鼠抑郁样行为学表现。方法对15只约9周龄C57BL/6雌性小鼠进行白癜风动物模型的构建,通过肉眼观察以及第23天采用表皮全层免疫荧光染色检测鼠尾组织以判断白癜风小鼠模型构建成功与否;在第8天(成模前)和第21天(成模后早期)采用高架十字迷宫实验和旷场实验检测小鼠行为学表现,包括进入开臂次数、开臂停留时间百分比、中央区域停留时间百分比和总行进距离,以评估白癜风小鼠模型是否出现抑郁样行为。为进一步明确白癜风造模对小鼠抑郁样状态影响的严重程度,将20只C57BL/6雌性小鼠平均分为单纯白癜风造模组和白癜风造模+慢性束缚应激组,其中白癜风造模+慢性束缚应激组小鼠在第9天开始进行慢性束缚应激,即置于离心管中,每天束缚约6 h,持续28 d;两组均在白癜风造模后第7、22、29和38天采用高架十字迷宫实验和旷场实验检测小鼠上述行为学指标。单组前后2次重复测量数据的比较采用配对t检验,多个时间点重复测量资料的比较采用两因素重复测量的方差分析。结果肉眼观察造模小鼠尾部皮肤逐渐出现境界清楚的白斑,与白癜风患者的临床表现相似,同时第23天鼠尾表皮全层免疫荧光染色、激光共聚焦显微镜下观察,见明显的CD8^(+)T细胞浸润,且Melan-A阳性的表皮黑素细胞数量减少,具有典型的白癜风病理特征,提示白癜风小鼠模型构建成功,第23天成功建模12只。高架十字迷宫实验显示,这12只白癜风模型小鼠第21天进入开臂次数[(2.33±1.78)次]、开臂停留时间百分比(5.01%±5.27%)均明显低于第8天[(10.75±2.30)次,29.20%±12.48%;t=9.63、6.36,均P<0.001];旷场实验显示,第21天在中央区域停留时间百分比(2.31%±1.53%)和总行进距离[(2518.31±528.38)cm]明显低于第8天[4.47%±2.65%、(3533.45±465.47)cm;t=2.40、5.47,P=0.036、<0.001]。慢性束缚应激实验中,第23天检测�Objective To observe and analyze depression-like behavioral performances of mouse models of vitiligo.Methods Fifteen female C57BL/6 mice aged about 9 weeks were modeled for vitiligo.Whether the mouse models of vitiligo were successfully constructed or not was determined by macroscopy and full-thickness epidermal immunofluorescence staining of mouse tail tissues on day 23 after the start of the experiment;on day 8(pre-modeling stage)and day 21(early modeling stage),the elevated plus maze test and the open field test were used to evaluate the behavioral performances of the mice,including the number of entry into the open arms,percentages of time spent in the open arms,percentages of time spent in the central area and total distance traveled,aiming to assess whether depression-like behaviors were exhibited in the mouse models of vitiligo.To further clarify the degree of the impact of vitiligo modeling on the depression-like state in mice,20 female C57BL/6 mice were equally divided into 2 groups:vitiligo modeling group and vitiligo modeling+chronic restraint stress group;the mice in the vitiligo modeling+chronic restraint stress group were subjected to chronic restraint stress on day 9,that is,these mice were placed in centrifuge tubes and restrained for about 6 hours every day for 28 consecutive days;on days 7,22,29 and 38 after the start of vitiligo modeling,the above-mentioned behavioral indicators were determined by the elevated plus maze test and open field test in the 2 groups.Repeated measurement data in a single group were compared before and after treatment by using paired t-test,and repeated measurement data at multiple time points were compared by using two-way repeated measures analysis of variance.Results By macroscopy,the mice gradually developed well-defined white patches on the tail skin during vitiligo modeling,which were similar to the clinical manifestations of vitiligo patients;on day 23,full-thickness epidermal immunofluorescence staining of the mouse tail tissues was conducted and showed obvious

关 键 词：白癜风 疾病模型 动物 抑郁 行为学表现 慢性束缚应激 高架十字迷宫实验 旷场实验 

分 类 号：R758.41[医药卫生—皮肤病学与性病学] R-332[医药卫生—临床医学]