一测多评法同时测定天麻头风灵胶囊中10种成分的含量  

Simultaneous content determination of ten constituents in Tianma Toufengling Capsules by QAMS

作　　者：郭绪生 邱学伟 李丽[1,2] 吴爱英 陈安珍 刘红兵 卢京光[1,2,3] GUO Xu-sheng;QIU Xue-wei;LI Li;WU Ai-ying;CHEN An-zhen;LIU Hong-bing;LU Jing-guang(Ocean University of China,Qingdao 266000,China;Key Laboratory for Quality Research and Evaluation of Traditional Marine Chinese Medicine,Qingdao 266000,China;Qingdao Municipal Institute for Food and Drug Control,Qingdao 266000,China)

机构地区：[1]中国海洋大学,山东青岛266000 [2]海洋中药质量研究与评价重点实验室,山东青岛266000 [3]青岛市食品药品检验研究院,山东青岛266000

出　　处：《中成药》2024年第2期359-364,共6页Chinese Traditional Patent Medicine

基　　金：国家药典委员会《中国药典》药品标准提高任务(WX-17-26)。

摘　　要：目的建立一测多评法同时测定天麻头风灵胶囊中天麻素、巴利森苷E、紫丁香苷、巴利森苷B、巴利森苷C、阿魏酸、巴利森苷A、蒙花苷、哈巴俄苷、肉桂酸的含量。方法分析采用GL Science Inertsil^(TM)ODS-3色谱柱(150 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长220、280 nm。以紫丁香苷为内标,计算其他9种成分的相对校正因子,测定其含量。结果10种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率98.53%~102.22%,RSD 1.26%~2.68%。一测多评法所得结果与外标法接近。结论该方法准确、专属性强,可用于天麻头风灵胶囊的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of gastrodin,parishin E,syringin,parishin B,parishin C,ferulic acid,parishin A,buddleoside,harpagoside and cinnamic acid in Tianma Toufengling Capsules.METHODS The analysis was performed on a 30℃thermostatic GL Science Inertsil^(TM)ODS-3 column(150 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,280 nm.Syringin was used as an internal standard to calculate the relative correction factors of the other nine constituents,after which the content determination was made.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.9997),whose average recoveries were 98.53%-102.22%with the RSDs of 1.26%-2.68%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate and specific method can be used for the quality control of Tianma Toufengling Capsules.

关 键 词：天麻头风灵胶囊 化学成分 含量测定 一测多评 

分 类 号：R927.2[医药卫生—药学]