Self-confocal NIR-II fluorescence microscopy for multifunctional in vivo imaging  

作　　者：Jing Zhou Tianxiang Wu Runze Chen Liang Zhu Hequn Zhang Yifei Li Liying Chen Jun Qian 

机构地区：[1]State Key Laboratory of Modern Optical Instrumentations,Centre for Optical and Electromagnetic Research,College of Optical Science and Engineering,International Research Center for Advanced Photonics,Zhejiang University,Hangzhou 310058,P.R.China [2]Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine,Zhejiang University,Hangzhou 310058,P.R.China [3]College of Biomedical Engineering and Instrument Science,Interdisciplinary Institute of Neuroscience and Technology(ZIINT),Zhejiang University,Hangzhou 310027,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2024年第1期105-119,共15页创新光学健康科学杂志（英文）

基　　金：supported by National Natural Science Foundation of China(61975172,82001874 and 61735016).

摘　　要：Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.

关 键 词：Self-confocal fiber-pinhole air-pinhole multi-channe fluorescence lifetime imaging multi-color imaging 

分 类 号：TH742[机械工程—光学工程]