基于JAK2/STAT3信号通路的乌鸡肽营养素配方治疗贫血功效研究  

Study on efficacy of black-bone silky fowl peptide nutrient formula based on JAK2/STAT3 signaling pathway in treatment of anemia

作　　者：张卓然 廖群 冯志远 刘丹 李明亮 李诒光 任杰 许锦珍 刘文颖 谷瑞增 刘文君 ZHANG Zhuoran;LIAO Qun;FENG Zhiyuan;LIU Dan;LI Mingliang;LI Yiguang;REN Jie;XU Jinzhen;LIU Wenying;GU Ruizeng;LIU Wenjun(Beijing Engineering Research Center of Protein&Functional Peptides,China National Research Institute of Food and Fermentation Industries,Beijing 100015,China;Jiangzhong Pharmaceutical Co.Ltd.,Nanchang 330096,China)

机构地区：[1]中国食品发酵工业研究院有限公司,北京市蛋白功能肽工程技术研究中心,北京100015 [2]江中药业股份有限公司,江西南昌330096

出　　处：《食品与发酵工业》2024年第3期80-85,共6页Food and Fermentation Industries

基　　金：宁夏回族自治区重点研发计划项目(2021BEG02027)。

摘　　要：乌鸡是我国特有的鸡种,具有很好的补血功能,利用乌鸡开发新的补血产品具有很大优势。该研究通过对贫血小鼠灌喂添加营养素(EDTAFeNa和维生素)的乌鸡肽配方样品,测试对其造血能力的恢复功效并通过探究信号通路研究造血作用机制。实验通过使用环磷酰胺和乙酰苯肼制造小鼠贫血模型,并检测外周血象、骨髓病理学观察、间接胆红素含量、ATP酶活力、造血因子水平以及JAK2/STAT3信号通路。结果表明乌鸡肽营养素配方可以显著恢复外周血细胞和骨髓造血干细胞数量;减少间接胆红素含量,提高ATP酶活力从而保护红细胞免受破裂损伤;通过分泌促红细胞生成素(erythropoietin,EPO)、重组巨噬细胞粒细胞集落刺激因子(human granulocyte-macrophage colony stimulating factor,GM-CSF)和白细胞介素-3(interleukin-3,IL-3)造血因子促进造血细胞恢复,并通过JAK2/STAT3信号通路调控造血细胞增殖分化。该研究为乌鸡肽的开发和利用提供了指导。As a unique species of chicken in China,the black-bone silky fowl has excellent haemopoietic properties.The black-bone silky fowl has been utilized to great advantage in the development of new blood supplement products.This study aimed to produce a sample of black-bone silky fowl peptide formula supplemented with the nutrients(EDTAFeNa,vitamins B 1,B 6,B 12,and folic acid).The efficacy of the sample in restoring haematopoiesis was tested by feeding them to mice and the mechanism of haematopoiesis was investigated.The anemia model of mice was made by using cyclophosphamide and acetylphenylhydrazine,and the peripheral blood picture,bone marrow pathological observation,indirect bilirubin content,ATPase activity,hematopoietic factor level,and JAK2/STAT3 signaling pathway were detected.Results showed that black-bone silky fowl peptide nutrient formula could significantly restore peripheral blood cell and bone marrow haematopoietic stem cell populations.It reduced indirect bilirubin levels and increased ATPase activity,thus protecting red blood cells from rupture damage.It promoted hematopoietic cell recovery through the secretion of erythropoietin(EPO),human granulocyte-macrophage colony-stimulating factor(GM-CSF),and interleukin-3(IL-3)hematopoietic factors,and regulated hematopoietic cell proliferation and differentiation through the JAK2/STAT3 signaling pathway.The study provided guidance for the development and utilization of black-bone silky fowl peptide.

关 键 词：乌鸡肽 贫血 环磷酰胺 乙酰苯肼 JAK2/STAT3信号通路 造血能力 

分 类 号：TS251.55[轻工技术与工程—农产品加工及贮藏工程]