航空食品生产环境中肠杆菌科细菌的分离鉴定及多位点序列分型研究  

Isolation and Identification of Enterobacteriaceae Bacteria in Aviation Food Production Environment and Study on Multi Locus Sequence Typing

作　　者：邬汶 杨雨 张青 邓晓东 高国龙 刘杨 胡政泽 Wu Wen;Yang Yu;Zhang Qing;Deng Xiaodong;Gao Guolong;Liu Yang;HuZhengzei(School of Public Health,Chengdu Medical College,Chengdu Sichuan 610500,China;Sichuan International Travel Health Care Center(Port Outpatient Department of Chengdu Customs),Chengdu Sichuan 610041,China;Sichuan Provincial Key Laboratory for Epidemic Monitoring at Ports,Chengdu Sichuan 610041,China;Tianjin International Travel Health Tianjin Customs Port Outpatient Department,Tianjin 300450,China)

机构地区：[1]成都医学院公共卫生学院,四川成都610500 [2]四川国际旅行卫生保健中心(成都海关口岸门诊部),四川成都610041 [3]口岸疫病疫情监测四川省重点实验室,四川成都610041 [4]天津国际旅行卫生保健中心(天津海关口岸门诊部),天津300450

出　　处：《口岸卫生控制》2024年第1期51-55,58,共6页Port Health Control

基　　金：成都海关自立项目(2022CK003)。

摘　　要：目的对航空食品生产环境中肠杆菌科细菌进行分离鉴定和多位点序列分型研究,了解生产环境中肠杆菌科细菌的污染状况和来源。方法采集航空食品生产车间的操作台、推车和地面等32份环境涂抹样品,从中分离肠杆菌科细菌,采用生化实验和16S rDNA测序方法鉴定细菌种类,并进行多位点序列分型。结果从32份环境涂抹样品中分离出7株肠杆菌科细菌,包括3株产酸克雷伯菌、2株肺炎克雷伯菌、1株阴沟肠杆菌、1株河生肠杆菌。3株产酸克雷伯菌多位点序列分型的型别为ST126、ST160和ST277。结论本研究未从航空食品生产环境中分离到致病菌,但分离到肺炎克雷伯菌和产酸克雷伯菌、阴沟肠杆菌、河生肠杆菌等条件致病菌,这些条件致病菌对食品的污染风险也应引起重视。Objective To isolate and identify Enterobacteriaceae bacteria in the aviation food production environment,and study their multi locus sequence typing,in order to understand the pollution status and sources of Enterobacteriaceae bacteria.Methods 32 environmental smear samples from the operating platform,trolley,and ground of the aviation food production workshop were collected and Enterobacteriaceae bacteria from these smear samples was isolated.By using biochemical experiments and 16S rDNA sequencing methods,bacterial species were identified and multi site sequence typing was performed.Results Seven strains of Enterobacteriaceae bacteria were isolated from 32 environmental smear samples,including 3 strains of Klebsiella oxytoca,2 strains of Klebsiella pneumoniae,1 strain of Enterobacter cloacae,and 1 strain of Lelliottia amnigena.The multi locus sequence typing of 3 strains of Klebsiella oxytoca are ST126,ST160,and ST277.Conclusion No pathogenic bacteria was isolated from the aviation food production environment in this study,but conditional pathogens such as Klebsiella pneumoniae,Klebsiella oxytoca,Enterobacter cloacae,and Lelliottia amnigena were isolated.The risk of food contamination by these conditional pathogens should also be taken seriously.

关 键 词：肠杆菌科细菌 16S rDNA测序 多位点序列分型 产酸克雷伯菌 

分 类 号：R372[医药卫生—病原生物学]