Long noncoding RNA protein-disulfide isomerase-associated 3 regulated high glucose-induced podocyte apoptosis in diabetic nephropathy through targeting miR-139-3p  

作　　者：Yin-Xi He Ting Wang Wen-Xian Li Yan-Xia Chen 

机构地区：[1]Department of Orthopaedic Trauma,The Third Hospital of Shijiazhuang,Shijiazhuang 050000,Hebei Province,China [2]Department of Endocrinology,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China [3]Department of Endocrinology,The First Hospital of Zhangjiakou,Zhangjiakou 075000,Hebei Province,China

出　　处：《World Journal of Diabetes》2024年第2期260-274,共15页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by the Natural Science Funds for Young Scholar of Hebei,China,No.H2020206108;the Subject of Health Commission of Hebei,China,No.20210151.

摘　　要：BACKGROUND Podocyte apoptosis plays a vital role in proteinuria pathogenesis in diabetic nephropathy(DN).The regulatory relationship between long noncoding RNAs(lncRNAs)and podocyte apoptosis has recently become another research hot spot in the DN field.AIM To investigate whether lncRNA protein-disulfide isomerase-associated 3(Pdia3)could regulate podocyte apoptosis through miR-139-3p and revealed the underlying mechanism.METHODS Using normal glucose or high glucose(HG)-cultured podocytes,the cellular functions and exact mechanisms underlying the regulatory effects of lncRNA Pdia3 on podocyte apoptosis and endoplasmic reticulum stress(ERS)were explored.LncRNA Pdia3 and miR-139-3p expression were measured through quantitative real-time polymerase chain reaction.Relative cell viability was detected through the cell counting kit-8 colorimetric assay.The podocyte apoptosis rate in each group was measured through flow cytometry.The interaction between lncRNA Pdia3 and miR-139-3p was examined through the dual luciferase reporter assay.Finally,western blotting was performed to detect the effect of lncRNA Pdia3 on podocyte apoptosis and ERS via miR-139-3p.RESULTS The expression of lncRNA Pdia3 was significantly downregulated in HG-cultured podocytes.Next,lncRNA Pdia3 was involved in HG-induced podocyte apoptosis.Furthermore,the dual luciferase reporter assay confirmed the direct interaction between lncRNA Pdia3 and miR-139-3p.LncRNA Pdia3 overexpression attenuated podocyte apoptosis and ERS through miR-139-3p in HG-cultured podocytes.CONCLUSION Taken together,this study demonstrated that lncRNA Pdia3 overexpression could attenuate HG-induced podocyte apoptosis and ERS by acting as a competing endogenous RNA of miR-139-3p,which might provide a potential therapeutic target for DN.

关 键 词：Long noncoding RNAs Diabetic nephropathy Podocyte apoptosis Endoplasmic reticulum stress Competing endogenous RNA 

分 类 号：R587.2[医药卫生—内分泌]