Urinary metabolic profiles during Helicobacter pylori eradication in chronic gastritis  

作　　者：Wen-Ting An Yu-Xia Hao Hong-Xia Li Xing-Kang Wu 

机构地区：[1]Department of Pharmacy,Shanxi Provincial People’s Hospital,Taiyuan 030012,Shanxi Province,China [2]Department of Gastroenterology,Shanxi Provincial People's Hospital,Taiyuan 030012,Shanxi Province,China [3]Department of Oncology,Shanxi Provincial People’s Hospital,Taiyuan 030012,Shanxi Province,China [4]Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,Shanxi Province,China

出　　处：《World Journal of Clinical Cases》2024年第5期951-965,共15页世界临床病例杂志

基　　金：Supported by Shanxi Provincial Health Commission,No.2019022.

摘　　要：BACKGROUND Helicobacter pylori(H.pylori)infection is a major risk factor for chronic gastritis,affecting approximately half of the global population.H.pylori eradication is a popular treatment method for H.pylori-positive chronic gastritis,but its mecha-nism remains unclear.Urinary metabolomics has been used to elucidate the mechanisms of gastric disease treatment.However,no clinical study has been conducted on urinary metabolomics of chronic gastritis.AIM To elucidate the urinary metabolic profiles during H.pylori eradication in patients with chronic gastritis.METHODS We applied LC–MS-based metabolomics and network pharmacology to in-vestigate the relationships between urinary metabolites and H.pylori-positive chronic gastritis via a clinical follow-up study.RESULTS Our study revealed the different urinary metabolic profiles of H.pylori-positive chronic gastritis before and after H.pylori eradication.The metabolites regulated by H.pylori eradication therapy include cis-aconitic acid,isocitric acid,citric acid,L-tyrosine,L-phenylalanine,L-tryptophan,and hippuric acid,which were involved in four metabolic pathways:(1)Phenylalanine metabolism;(2)phenylalanine,tyrosine,and tryptophan biosynthesis;(3)citrate cycle;and(4)glyoxylate and dicarboxylate metabolism.Integrated metabolomics and network pharmacology revealed that MPO,COMT,TPO,TH,EPX,CMA1,DDC,TPH1,and LPO were the key proteins involved in the biological progress of H.pylori eradication in chronic gastritis.CONCLUSION Our research provides a new perspective for exploring the significance of urinary metabolites in evaluating the treatment and prognosis of H.pylori-positive chronic gastritis patients.

关 键 词：LC-MS metabolomics chronic gastritis Helicobacter pylori urinary metabolites 

分 类 号：R725.7[医药卫生—儿科]