黄百香果茎段组培快繁技术研究  

Research on Tissue Culture and Rapid Propagation of Stem Segments of Yellow Passion Fruit

作　　者：刘洁云[1] 张英俊 牟海飞[1] 吴艳艳[1] 黄伟华[1] 田青兰 韦毅刚[2] 温放[2] LIU Jieyun;ZHANG Yingjun;MOU Haifei;WU Yanyan;HUANG Weihua;TIAN Qinglan;WEI Yigang;WEN Fang(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi,530007,China;Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin,Guangxi,541006,China)

机构地区：[1]广西壮族自治区农业科学院,南宁530007 [2]广西壮族自治区/中国科学院广西植物研究所,广西桂林541006

出　　处：《中国南方果树》2024年第1期44-49,55,共7页South China Fruits

基　　金：广西科技重大专项(桂科AA22068091、桂科AA22068091-3);国家自然科学基金(32260740、32060660);广西农业科学院稳定资助科研团队项目(桂农科2021YT089);广西农业科学院基本科研业务专项(桂农科2017YM31)资助;科技先锋队“强农富民”“六个一”专项行动(桂农科盟202204-05)。

摘　　要：为筛选黄百香果组培快繁方案,为百香果组培苗繁育提供技术支撑,以黄百香果茎段为外植体,研究不同灭菌时间对外植体成活率的影响,不同培养基配方对初代芽诱导、继代增殖、生根移栽的影响。结果表明,0.1%HgCl_(2)处理6 min对百香果茎段外植体的灭菌效果最佳,外植体成活率为66.67%。MS+6-苄氨基嘌呤(6-BA)0.3 mg/L+吲哚丁酸(IBA)0.1 mg/L+抗坏血酸300 mg/L为芽诱导最佳培养基,芽诱导率79.14%。MS+6-BA 0.2 mg/L+IBA 0.05 mg/L+谷氨酸20 mg/L为继代增殖最佳培养基,增殖倍数3.21。MS+IBA 0.5 mg/L生根培养基的组培苗生根率100%,且有利于组培生根苗移栽成活。To screen the rapid propagation scheme of passion fruit by tissue culture and provide technical support for the breeding of passion fruit tissue culture seedlings,the effects of different sterilization times on the survival rate of explants and different medium compositions on bud induction,subculture proliferation,rooting and transplanting were studied using stem segments of yellow passion fruit as explants.The results showed that treatment of 0.1%HgCl_(2)for 6 minutes had the best sterilization effect on passion fruit stem explants,with a survival rate of 66.67%.The culture medium of MS+0.3 mg·L^(-1)6-benzylaminopurine(6-BA)+0.1 mg·L^(-1)indole-3-butyric acid(IBA)+300 mg·L^(-1)ascorbic acid was the optimal medium for bud induction with the bud induction rate of 79.14%.The best subculture proliferation medium was MS+0.2 mg·L^(-1)6-BA+0.05 mg·L^(-1)IBA+20 mg·L^(-1)glutamic acid,with a proliferation rate of 3.21.The MS+0.5 mg·L^(-1)IBA was the optimal rooting medium with a 100%rooting rate,and was conducive to the survival of the transplanted tissue culture seedlings.

关 键 词：黄百香果 茎段 HgCl2 芽诱导 增殖倍数 生根率 

分 类 号：S667.9[农业科学—果树学]