Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction  

作　　者：Di Xie Zhulan Liu Yunfeng Cao Sheng-I Yang Chen Su Mi Li 

机构地区：[1]Jiangsu Co-innovation Center for Efficient Processing and Utilization of Forest Products,Nanjing Forestry University,Nanjing 210037,China [2]International Innovation Center for Forest Chemicals and Materials,Nanjing Forestry University,Nanjing 210037,China [3]Center for Renewable Carbon,School of Natural Resources,University of Tennessee,Knoxville,TN 37996,USA [4]Shandong Huatai Paper Co.,Ltd.,Dongying 257000,China [5]School of Natural Resources,University of Tennessee,Knoxville,TN 37996,USA [6]Key Lab of Biomass Energy and Material,Institute of Chemical Industry of Forest Products,Chinese Academy of Forestry,Jiangsu Province,Nanjing 210042,China

出　　处：《Journal of Bioresources and Bioproducts》2024年第1期113-125,共13页生物质资源与工程（英文）

基　　金：support by National Natural Science Foundation of China (No.31670591);the fellowship of China Postdoctoral Science Foundation (No.2022M711229);Natural Science Foundation of Jiangsu Province (No.BK20160928);supported by the South Eastern Regional Sun Grant Centre and AgResearch at the University of Tennessee.

摘　　要：Water-soluble lignin-carbohydrate complex(LCC)rich in polysaccharides exhibits benign in vitro antioxidant activities and distinguishes high biocompatibility from lignin-rich LCC and lignin.However,the antioxidant activity of water-soluble LCCs remains to be improved and its structure-antioxidant relationship is still uncertain.Herein,structurally diversified water-soluble LCCs were isolated under different ball-milling pretreatment durations(4,6,8 h),extraction pathways(ho-mogeneous and heterogeneous),and isolation routines(water extracts and residues after water extraction).Their structures were characterized by wet chemistry,chromatography and spec-troscopies.Antioxidant activities were evaluated by ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl radicals scavenging rate(RDPPH).Results show that altering ball-milling duration and isolation procedures cause varied structures and antioxidant activities of the water-soluble LCCs.Specifically,prolonging ball-milling duration to 8 hours and homogeneous extrac-tion can enhance their antioxidant activity through releasing more phenolic structures and pro-moting the extraction of high-molecular-weight LCCs via reducing mass-transfer resistance,re-spectively.As a result,the RDPPH of water-soluble LCCs reaches up to 97.35%,which is associated with the arabinan content with statistical significance(P<0.05).This study provides new insights into the structure-antioxidation relationship of herbaceous LCC as potential antioxidants.

关 键 词：LCC Homogeneous extraction Ball-milling Structure-antioxidation relationship Wheat straw 

分 类 号：O6[理学—化学]