红榄李来源内生真菌Penicillium oxalicum HLLG-13次级代谢产物与生物活性研究  

Study on the Secondary Metabolites and Biological Activity from Lumnitzera littorea-Derived Endophytic Fungus Penicillium oxalicum HLLG-13

作　　者：王越 张玉琴[3] 徐喆菲 白琪琪 陈文豪[1,2] 周学明 陈光英[1,2] WANG Yue;ZHANG Yuqin;XU Zhefei;BAI Qiqi;CHEN Wenhao;ZHOU Xueming;CHEN Guangying(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571127,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,Haikou 571127,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]海南师范大学化学与化工学院,热带药用资源化学教育部重点实验室,海南海口571127 [2]海南省热带药用植物化学重点实验室,海南海口571127 [3]福建中医药大学药学院,福建福州350122

出　　处：《海南师范大学学报（自然科学版）》2023年第4期420-428,共9页Journal of Hainan Normal University（Natural Science）

基　　金：国家自然科学基金项目(22177023,41866005);海南省自然科学基金项目(221RC1054);海南省重大科技计划项目(ZDKJ202008);中央引导地方科技发展资金项目(ZY2022HN08)。

摘　　要：对一株红树植物红榄李(Lumnitzera littorea (Jacq.) Voigt)内生真菌Penicillium oxalicum HLLG-13次级代谢产物进行系统研究;综合运用多种色谱分离方法结合波谱、光谱技术从中分离鉴定了21个单体化合物,分别为2-(4-羟苯基)乙基-(2S)-羟基丙酸(1),2-(4-羟苯基)乙酸乙酯(2),(4-羟苯基)乙酸甲酯(3),4-羟基苯乙醇(4),2,4,5-三甲基间苯二酚(5),1-(2,6-dihydroxyphenyl) butan-1-one(6),rosmarinosin C(7),penicyclone A(8),penicyclone C(9),2-(N-乙酰氨基)苯酚(10),N-乙酰酪胺(11),2-[[(2S)-羟基-1-氧丙基]氨基]苯甲酰胺(12),peniamidone A(13),meleagrin(14),7-羟基-2-(2’S-羟丙基)-5-甲基色酮(15),3-甲基-6,8-二羟基异香豆素(16),(-)-6-hydroxymellein(17),(3R)-甲基-5-氯-6,8-二羟基-二氢异香豆素(18),(3R)-甲基-6,8-二羟基-7-甲氧基-二氢异香豆素(19),(-)-citreoisocoumarinol(20)和de-O-methyldiaporthin(21)。抗菌活性测试结果表明,所有化合物对测试的5株人体致病菌和3株弧菌均无明显抗菌活性;神经保护活性测试结果表明,化合物16具有中等的神经保护活性,在200μmol/L浓度下,可以将H2O_(2)氧化损伤的HT22细胞存活率由43.36%提升至60.15%。Twenty-one compounds were isolated from the EtOAc extract of the fermentation broth of the Lumnitzera litto⁃rea-derived fungus Penicillium oxalicum HLLG-13.Their structures were elucidated by 1D NMR,MS spectral analyses and compared with literature data.The compounds were identified as 2-(4-hydroxyphenyl)ethyl(2S)-2-hydroxypropanoate(1),2-(4-hydroxyphenyl)ethylacetate(2),methyl(4-hydroxyphenyl)acetate(3),4-hydroxybenzeneethanol(4),2,4,5-tri⁃methylresorcinol(5),1-(2,6-dihydroxyphenyl)butan-1-one(6),rosmarinosin C(7),penicyclone A(8),penicyclone C(9),2-(N-acetylamino)phenol(10),N-acetyltyramine(11),2-[[(2S)-2-hydroxy-1-oxopropyl]amino]benzamide(12),peniami⁃done A(13),meleagrin(14),7-hydroxy-2-(2'S-hydroxypropyl)-5-methylchromone(15),6,8-dihydroxy-3-methylisocou⁃marin(16),(-)-6-hydroxymellein(17),(3R)-5-chloro-3,4-dihydro-6,8-dihydroxy-3-methyl-1H-2-benzopyran-1-one(18),(3R)-3,4-dihydro-6,8-dihydroxy-7-methoxy-3-methyl-1H-2-benzopyran-1-one(19),(-)-citreoisocoumarinol(20)and de-O-methyldiaporthin(21).All compounds exhibited no antibacterial activity against the five human pathogenic bacterium and three vibrios.Compound 16 showed moderate neuroprotective activity,which could increase the cell survival rate of H2O_(2) induced oxidative damage HT22 cells from 43.36%to 60.15%at the concentration of 200µmol/L.

关 键 词：红榄李 Penicillium oxalicum 次级代谢产物 抗菌活性 神经保护活性 

分 类 号：R284.1[医药卫生—中药学]