694家医疗机构性病实验室沙眼衣原体室间质评结果分析  

Evaluation of the ability of STD laboratories to detect Chlamydia trachomatis in Guangdong province

作　　者：覃晓琳 陈洋 薛耀华 徐青青 欧江丽 许巧珍 吴兴中 陈文韬 尹笑纳 周涵 张自岩 刘丹 黄进梅 郑和平 QIN Xiaolin;CHEN Yang;XUE Yaohua;XU Qingqing;OU Jiangli;XU Qiaozhen;WU Xingzhong;CHEN Wentao;YIN Xiaona;ZHOU Han;ZHANG Ziyan;LIU Dan;HUANG Jinmei;ZHENG Heping(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China;Guangzhou Key Laboratory of Precise Prevention and Control of Sexually Transmitted Diseases,Guangzhou 510091,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091 [2]广州市性传播疾病精准防控重点实验室,广东广州510091

出　　处：《皮肤性病诊疗学杂志》2023年第6期544-548,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：国家自然科学基金(81974307);广州市科技计划项目(202201000007)。

摘　　要：目的 评估广东省不同医疗机构性病实验室沙眼衣原体检测能力。方法 统一制备并发放沙眼衣原体检测样本,各实验室按照日常检测方法对沙眼衣原体进行检测,通过省性病实验室管理信息系统上报检测方法、试剂品牌和结果等,结果与靶值一致得分,不一致不得分。比较不同性质医疗机构、不同类型性病实验室以及不同检测方法的检测结果。结果 全省共694家医疗机构实验室参加本次室间质评并回报结果,包括388家二级及以上级别医疗机构、82家妇幼机构、79家皮防慢病机构、145家一级或其他医疗机构。其中规范化性病实验室537家、普通性病实验室157家。总体符合率96.11%,合格率98.70%。皮防慢病机构结果符合率最高,为98.23%;规范化性病实验室得分(Z=4.25)和合格率(χ^(2)=22.87)均显著高于普通性病实验室(P<0.001)。核酸检测方法得分显著高于免疫层析快速法(Z=3.72,P<0.001)。结论 广东省沙眼衣原体实验室检测能力处于较高水平。皮防慢病机构作为性病防治机构,具有更高的检测符合率;规范化性病实验室比普通性病实验室具有更高的检测能力。加强性病实验室规范化建设可有效促进实验室检测能力的提高。临床应加强沙眼衣原体核酸检测方法的使用。Objective To evaluate the ability of sexually transmitted disease(STD)laboratories in different medical institutions to detect Chlamydia trachomatis in Guangdong province.Methods The samples of Chlamydia trachomatis were prepared and distributed to all participants,without specified detection methods.The test results,methods and reagents were reported through the EQA online reporting system of provincial STD laboratory.If the results agreed with the expected results,a score of 2 would be achieved.Otherwise,the score was zero.The test results from different medical institutions,the types of STD laboratories and testing methods were compared.Results A total of 694 laboratories,including 388 secondary and higher-level medical institutions,82 maternal and child health institutions,79 chronic disease prevention institutions,and 145 primary or other medical institutions,participated in the evaluation of the inter-laboratory ability.Of the 694 laboratories,537 were standardized STD laboratories and 157 were general laboratories.The overall conformity rate was 96.11%,with a qualification rate of 98.70%.Notably,the conformity rate of laboratories in chronic disease prevention institutions was the highest(98.23%).The standardized STD laboratories achieved significantly higher scores(Z=4.25)and qualification rates(χ^(2)=22.87)than non-standardized laboratories(both P<0.001).Additionally,the nucleic acid testing method yielded a significantly higher score than the immunochromatographic method(Z=3.72,P<0.001).Conclusions The ability to detect Chlamydia trachomatis is high in the STD laboratories in Guangdong province.As the institutions dedicated to the prevention and treatment of STDs,skin venereal disease prevention and control institutions show a higher conformity rate.Moreover,standardized STD laboratories demonstrate higher detection ability than the non-standardized STD laboratories.Therefore,strengthening the standardization of STD laboratories can effectively improve the detection ability.Meanwhile,the nucleic acid t

关 键 词：性病实验室 沙眼衣原体 检测 能力 评价 

分 类 号：R759[医药卫生—皮肤病学与性病学]