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作 者:陈金华 Chen Jinhua(Zhanggang Sea Clam Farm,Changle District,Fuzhou 350209)
出 处:《福建畜牧兽医》2024年第1期24-29,共6页Fujian Journal of Animal Husbandry and Veterinary medicine
基 金:闽财农指[2023]32号项目资助。
摘 要:本文以海蚌育苗池海水中的溶藻弧菌作为研究对象,探讨过硫酸氢钾对溶藻弧菌的杀灭效果。通过单因素试验和3因素3水平正交试验探讨不同温度、不同过硫酸氢钾浓度、不同菌液浓度对抑制溶藻弧菌效果的影响。从单因素试验结果可知,当温度处于20~30℃、过硫酸氢钾浓度20~40 mg/L、菌液浓度10^(5)~10^(7)CFU/mL时,杀菌抑制效果显著;正交试验结果显示,温度25℃、过硫酸氢钾浓度40 mg/L、菌液浓度10^(5)CFU/mL,是过硫酸氢钾溶液对溶藻弧菌杀灭效果最佳的条件;药物浓度11 mg/L时,灭菌率≧99%;药物浓度大于9 mg/L时,药物作用时间1~24 h的杀菌率差异均不显著,过硫酸氢钾药效可以维持24 h。The killing effect of potassium monopersulfate on Vibrio alginolyticus in clam breeding ponds seawater was studied.The effects of different temperature,different concentration of potassium monopersulfate and different concentration of bacterial solution on the inhibition of Vibrio alginolyticus were studied by single factor experiment and 3 factor 3 orthogonal experiment.According to the results of single factor experiment,the germicidal effect was significant when the temperature was 20-30℃,the concentration of potassium monopersulfate was 20 mg/L-40 mg/L and the concentration of bacterial was 10^(5)-10^(7)CFU/mL;The orthogonal experiment results showed that the temperature of 25℃,potassium monopersulfate concentration of 40 mg/L and bacterial solution concentration of 10^(5)CFU/mL were the best conditions for killing Vibrio alginolyticus by potassium monopersulfate;When the drug concentration≧11 mg/L,the sterilization rate≧99%;When the drug concentration is greater than 9 mg/L,there is no significant difference in the bactericidal rate of the drug action time 1-24 h,and the efficacy of potassium persulfate can be maintained for 24 h.
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