大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响  

Characterization of groupⅠmetabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia

作　　者：魏茜茜 李超红[1,2] 赵晨露 唐家萍 刘玉珍[1,2] WEI Xi-xi;LI Chao-hong;ZHAO Chen-lu;TANG Jia-ping;LIU Yu-zhen(Department of Respiratory and Critical Care Medicine;Life Science Research Center,the First Affiliated Hospital of Xinxiang Medical University,He’nan Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院呼吸与危重症医学科,河南卫辉453100 [2]新乡医学院第一附属医院生命科学研究中心,河南卫辉453100

出　　处：《解剖学报》2024年第1期3-9,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(81973988);河南省神经修复重点实验室开放课题项目(HNSJXF⁃2021⁃014)。

摘　　要：目的观察Ⅰ组代谢型谷氨酸受体(mGluR1/5)在大鼠颈上神经节(SCG)组织的表达、定位,及慢性间歇性低氧(CIH)对mGluR1/5蛋白水平的影响。方法12只雄性SD大鼠随机分为对照组(Ctrl)和CIH组(CIH),每组6只,模型制作6周。Western blotting检测CIH对mGluR1/5蛋白水平的影响,免疫组织化学法和免疫荧光共定位染色检测大鼠SCG中mGluR1/5的表达及分布。结果mGluR1/5表达于大鼠SCG,mGluR1分布于神经元和小强荧光(SIF)细胞,而在卫星胶质细胞(SGCs)、神经纤维和血管不表达;mGluR5主要分布于神经纤维,少量分布于神经元,而在SIF细胞、SGCs及血管不表达;CIH上调mGluR1/5的蛋白水平(P<0.01)。结论mGluR1和mGluR5均表达于大鼠SCG,但其表达部位有所不同,两者蛋白水平的升高可能参与了CIH诱导的血压升高。Objective To observe the expression and localization of groupⅠmetabotropic glutamate receptors(mGluR1/5)in rat superior cervical ganglion(SCG)and the effect of chronic intermittent hypoxia(CIH)on mGluR1/5 protein level.Methods Twelve male SD rats were randomly divided into control group(Ctrl)and CIH group(CIH),6 rats in each group.After 6 weeks of modeling,the effect of CIH on mGluR1/5 protein level was detected by Western blotting,the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining.Results mGluR1/5 was expressed in rat SCG.mGluR1 was distributed in neurons and small intensely fluorescent(SIF)cells,but not in satellite glial cells(SGCs),nerve fibers and blood vessels,whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons,but not in SGCs,SIF cells and blood vessels.CIH increased the protein levels of mGluR1/5(P<0.01)in rat SCG.Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG,but their distribution are different,and the increased protein levels of both may be involved in CIH-induced hypertension.

关 键 词：Ⅰ组代谢型谷氨酸受体 颈上神经节 慢性间歇性低氧 高血压 免疫印迹法 大鼠 

分 类 号：R34[医药卫生—基础医学]