GC法同时测定复方樟脑酊中4种成分的含量  

Simultaneous Determination of 4 Constituents in Compound Camphor Tincture by GC

作　　者：孙乃霞 王瑞芬 郭社民 SUN Nai-xia;WANG Rui-fen;GUO She-min(Puyang Institute for Food and Drug Control,Puyang 457000,China)

机构地区：[1]河南省濮阳市食品药品检验检测中心,河南濮阳457000

出　　处：《食品与药品》2024年第1期67-70,共4页Food and Drug

摘　　要：目的 建立气相色谱法同时测定复方樟脑酊中乙醇、樟脑、八角茴香油和苯甲酸含量的方法。方法采用Agilent DA-INNOWax毛细管柱(30 m×0.25 mm×0.25μm),程序升温,进样口温度为220℃,氢火焰离子化检测器,检测器温度为250℃,载气为高纯氮气,载气流量1 ml/min,分流比20:1,进样量1μl。结果在该色谱条件下,乙醇、樟脑、八角茴香油和苯甲酸分别在106.65~533.25,0.1424~0.7118,0.1664~0.8318,0.2379~1.1895mg/ml范围内与对照品峰面积/内标峰面积呈良好的线性关系;平均加样回收率分别为99.8%,101.1%,98.6%,103.4%;RSD分别为1.5%,1.2%,1.8%,2.4%(n=9)。结论 该法准确方便,重现性高、稳定性好,可用于复方樟脑酊的质量控制。Objective To establish a GC method for the determination of ethanol,camphor,star anisi oil and benzoic acid in Compound Camphor Tincture.Methods An Agilent DA-INNOWax(30 m×0.25 mm×0.25μm)capillary column with programmed temperature was used.The inlet temperature was set at 220℃,and the FID detector temperature was set at 250℃.Nitrogen was used as the carrier gas,and the flow rate of nitogen was 1 ml/min.The spilt ratio was 20:1,and the volume of sample injection was 1μl.Results Under the above condition,ethanol,camphor,star anisi oil and benzoic acid had good linear relationships in the mass concentration range of 106.65-533.25,0.1424-0.7118,0.1664-0.8318,0.2379-1.1895 mg/ml,respectively.The average recoveries were 99.8%,101.1%,98.6%,103.4%,and RSDs(n=9)were 1.5%,1.2%,1.8%,2.4%,respectively.Conclusion The method is accurate,reproductive and stable,and can be used for quality control of Compound Camphor Tincture.

关 键 词：复方樟脑酊 乙醇 樟脑 八角茴香油 苯甲酸 

分 类 号：R927[医药卫生—药学]