中华枸杞中1个新的香豆素葡萄糖苷  

A new coumarin glucoside from fruits of Lycium chinense

作　　者：范雨欣 徐瑞雯 张晓祎 朱研洁 代向阳 孙彦君[1,2] 李孟 冯卫生[1,2] 陈辉 FAN Yuxin;XU Ruiwen;ZHANG Xiaoyi;ZHU Yanjie;DAI Xiangyang;SUN Yanjun;LI Meng;FENG Weisheng;CHEN Hui(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine Prevention and Treatment of Respiratory Diseases Co-construction by Henan Province&Ministry of Education,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中草药》2024年第1期23-29,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(22177027);河南省高校科技创新团队支持计划资助(24IRTSTHN039);河南省科技研发计划联合基金(优势学科培育类)项目(232301420067)。

摘　　要：目的研究中华枸杞Lycium chinense果实的化学成分。方法采用硅胶、D101大孔吸附树脂、ODS等柱色谱以及半制备液相色谱等现代分离技术进行分离纯化,根据化合物的波谱数据和理化性质进行结构鉴定,并对部分化合物进行α-葡萄糖苷酶抑制活性筛选。结果从中华枸杞醋酸乙酯部位分离得到11个化合物,分别鉴定为7-O-(6-O-3-羟基异丁酰基-β-D-吡喃葡萄糖基)-6-甲氧基香豆素(1)、东莨菪苷(2)、东莨菪素(3)、反式对香豆酰基-β-D-葡萄糖苷(4)、cassoside I(5)、对羟基苯乙酮-4-O-β-D-吡喃葡萄糖苷(6)、苯甲醇吡喃葡萄糖苷(7)、苯乙基-β-D-吡喃葡萄糖苷(8)、柚皮素-7-O-β-D-葡萄糖苷(9)、5-O-对香豆酰奎尼酸甲酯(10)、绿原酸甲酯(11)。结论化合物1为1个新的香豆素葡萄糖苷,命名为枸杞苷E。化合物10和11为首次从该属植物中分离得到。化合物4~11为首次从该植物中分离得到。化合物11对α-葡萄糖苷酶具有弱的抑制作用。Objective To isolate and identify the chemical constituents from the fruits of Lycium chinense.Methods The compounds were isolated and purified by column chromatography of silica gel,D101 macroporous resin,ODS,and semi-preparative HPLC,their structures were identified by spectroscopic data and physiochemical properties.Theα-glucosidase inhibitory activity of compounds was screened by PNPG assay.Results Eleven compounds were isolated from the ethyl acetate part of the fruits of L.chinense,which were elucidated as 7-O-(6-O-3-hydroxyisobutyroyl-β-D-glucopyranosyl)-6-methoxycoumarin(1),scopolin(2),scopoletin(3),trans-p-coumaroylβ-D-glucoside(4),cassoside I(5),4-hydroxyacetophenone-4-O-β-D-glucopyranoside(6),benzyl alcohol glucopyranoside(7),phenethyl-β-D-glucopyranoside(8),naringenin-7-O-β-D-glucoside(9),methyl 5-O-p-coumaroylquinic acid(10),methyl chlorogenate(11).Conclusion Compound 1 was a new coumarin glucoside,named lyciumoside E.Compounds 10 and 11 were isolated from Lycium genus for the first time.Compounds 4—11 were isolated from L.chinense for the first time.Compound 11 displayed weak inhibitory activity onα-glucosidase.

关 键 词：中华枸杞 香豆素葡萄糖苷 枸杞苷E 柚皮素-7-O-β-D-葡萄糖苷 5-O-对香豆酰奎尼酸甲酯 绿原酸甲酯 

分 类 号：R284.1[医药卫生—中药学]