维生素D受体基因多态性与RSV感染性毛细支气管炎的相关性研究  

Relationship between vitamin D receptor gene polymorphism and respiratory syncytial virus-induced bronchiolitis

作　　者：肖成伟[1] 胡金科 陈汉斌[1] XIAO Cheng-wei;HU Jin-ke;CHEN Han-bin(Department of Pediatric Critical Care Medicine,Foshan Maternal and Child Health Hospital,Foshan 528000,Guangdong,CHINA)

机构地区：[1]佛山市妇幼保健院儿童重症医学科,广东佛山528000

出　　处：《海南医学》2024年第3期334-338,共5页Hainan Medical Journal

基　　金：广东省佛山市自筹经费类科技创新计划项目(编号:2220001004498)。

摘　　要：目的探讨维生素D受体(VDR)基因多态性与呼吸道合胞病毒(RSV)感染性毛细支气管炎的相关性。方法回顾性分析2023年1~6月佛山市妇幼保健院收治的60例RSV感染性毛细支气管炎患儿的临床资料,根据患儿临床表现分为轻症组(n=37)和重症组(n=23),另选择同期30例RSV阴性的健康体检儿童作为对照组。分别采用酶联免疫吸附法检测三组儿童的血清25-羟基维生素D[25(OH)D]、白细胞介素4(IL-4)、白细胞介素5(IL-5)水平,采用限制性片段长度多态性方法分析维生素D受体TaqI、FokI、ApaI、Bsm I基因多态位点,并比较三组儿童的基因型及等位基因频率情况。结果重症组患儿的血清25(OH)D水平为(52.67±12.22)nmol/L,明显低于轻症组的(65.57±13.54)nmol/L,而轻症组又明显低于对照组的(82.57±15.30)nmol/L,IL-4、IL-5分别为(152.28±22.47)ng/L、(51.83±9.83)ng/L,明显高于轻症组的(130.48±21.35)ng/L、(44.72±8.70)ng/L,而轻症组又明显高于对照组的(77.48±15.26)ng/L、(26.52±7.78)ng/L,差异均有统计学意义(P<0.05);经Hardy-Weinberg平衡定律检测不同组别间VDR基因序列等位基因分布的频率,数据均符合平衡定律(P<0.05);重症组患儿的VDR TaqI(rs731236)基因型TT及等位基因C均高于轻症组和对照组,基因型TC、等位基因T型频率均低于轻症组和对照组,差异均有统计学意义(P<0.05),但三组基因型的CC频率分布比较差异无统计学意义(P>0.05);三组儿童的VDR FokI(rs2228570)基因型频率分布比较差异均无统计学意义(P>0.05),但重症组患儿的等位基因C高于轻症组和对照组,T型频率低于轻症组和对照组,差异均有统计学意义(P<0.05);重症组患儿的VDR ApaI(rs7975232)基因型GG及等位基因G高于轻症组和对照组,基因型GT及等位基因A频率分布低于轻症组和对照组,差异均有统计学意义(P<0.05);重症组患儿的VDR Bsm I(rs1544410)基因型GA、AA及等位基因A均高于轻症组和对照组,基因型GG及等Objective To explore the correlation between vitamin D receptor(VDR)gene polymorphism and respiratory syncytial virus(RSV)-induced bronchiolitis.Methods The clinical data of 60 children with RSV-induced bronchiolitis admitted to Foshan Maternal and Child Health Hospital from January 2023 to June 2023 were retrospectively analyzed.According to the clinical manifestations of the children,the patients were divided into a mild group(n=37)and a severe group(n=23).Another 30 healthy children who were RSV-negative in physical examination during the same period were selected as the control group.The serum levels of 25-hydroxyvitamin D[25(OH)D],interleukin-4(IL-4),and interleukin-5(IL-5)in the three groups were detected by enzyme-linked immunosorbent assay.The polymorphic sites of vitamin D receptors(TaqI,FokI,ApaI,and Bsm I gene)were analyzed by restriction fragment length polymorphism method.The genotype and allele frequency were compared among the three groups.Results The serum 25(OH)D level of children in the severe group was(52.67±12.22)nmol/L,which was significantly lower than(65.57±13.54)nmol/L of the mild group;the level in the mild group was significantly lower than(82.57±15.30)nmol/L of the control group;the IL-4 and IL-5 levels were(152.28±22.47)ng/L and(51.83±9.83)ng/L,which were significantly higher than(130.48±21.35)ng/L and(44.72±8.70)ng/L of the mild group,and the levels in the mild group were significantly higher than(77.48±15.26)ng/L and(26.52±7.78)ng/L of the control group;the differences were statistically significant(P<0.05).The distribution of allele frequencies of VDR gene sequences among different groups was detected by Hardy-Weinberg equilibrium law,and the data were in accordance with the equilibrium law(P<0.05).The VDR TaqI(rs731236)genotype TT and allele C in the severe group were higher than those in the mild group and the control group,and the genotype TC and allele T frequency were lower than those in the mild group and the control group,with statistically significant differences(P<

关 键 词：毛细支气管炎 呼吸道合胞病毒 维生素D受体 基因多态性 相关性 

分 类 号：R562.21[医药卫生—呼吸系统]