高敏丙型肝炎病毒核糖核酸在丙型肝炎中的诊断价值  

The clinical significance of high-sensitive hepatitis C virus-RNA on the diagnosis of hepatitis C

作　　者：戴倩梅 丁体龙[1] 代雪枫 于莉[2] 陈策[2] DAI Qianmei;DING Tilong△;DAI Xuefeng;YU Li;CHEN Ce(Central Laboratory,902nd People's Liberation Army Support Unit Hospital,Bengbu,Anhui 233015,China;Department of Infectious Disease,902nd People's Liberation Army Support Unit Hospital,Bengbu,Anhui 233015,China)

机构地区：[1]中国人民解放军联勤保障部队第九O二医院中心实验室,安徽蚌埠233015 [2]中国人民解放军联勤保障部队第九O二医院感染科,安徽蚌埠233015

出　　处：《检验医学与临床》2024年第4期487-490,共4页Laboratory Medicine and Clinic

基　　金：联勤保障部队第九O二医院医学科技创新计划项目(21MA004)。

摘　　要：目的比较高敏丙型肝炎病毒核糖核酸(HCV-RNA)与普通HCV-RNA、丙型肝炎病毒(HCV)核心抗原检测在丙型肝炎临床诊断中的优势,探讨高敏HCV-RNA在临床诊断和病情监测中的应用价值。方法选择48例HCV抗体阳性的门诊及住院患者作为观察组,另选择同期40例HCV抗体阴性的健康体检者作为对照组。采集所有研究对象5 mL空腹静脉血标本,留取血清。高敏HCV-RNA采用赛沛全自动医用聚合酶链反应(PCR)分析系统检测,普通HCV-RNA采用厦门安普利生物技术有限公司Anadas9850全自动核酸提纯系统及荧光定量PCR分析系统及配套试剂检测,HCV抗体及HCV核心抗原检测采用酶联免疫吸附试验。试验前,先对赛沛高敏HCV-RNA检测进行性能验证。结果高敏HCV-RNA检测性能验证准确度、重复性、线性范围和最低检出限符合要求。高敏HCV-RNA诊断丙型肝炎灵敏度明显高于普通HCV-RNA及HCV核心抗原检测。结论高敏HCV-RNA是丙型肝炎诊断的重要指标,并可用于丙型肝炎治疗终点判断和病情监测。Objective To compare the advantages of high sensitivity hepatitis C virus ribonucleic acid(HCV-RNA),common HCV-RNA and hepatitis C virus(HCV)core antigen detection in the clinical diagnosis of hepatitis C,and to explore the application value of HCV-RNA in clinical diagnosis and disease monitoring.Methods A total of 48 patients with positive HCV antibody were selected as the observation group,and 40 healthy patients with negative HCV antibody were selected as the control group.A total of 5 mL fasting venous blood samples were collected from all subjects and serum was retained.High-sensitive HCV-RNA was detected by Cepheid automatic medical polymerase chain reaction(PCR)analysis system,and common HCV-RNA was detected by Xiamen Anpli Biotechnology Co.,LTD.Anadas9850 automatic nucleic acid purification system,fluorescent PCR analysis system and supporting reagents.HCV antibodies and HCV core antigen were detected by enzyme-linked immunosorbent assay.Before the experiment,the performance of Cepheid high-sensitive HCV-RNA was verified.Results The accuracy,repeatability,linear range and minimum detection limit of high-sensitive HCV-RNA detection met the requirements.The sensitivity of high-sensitive HCV-RNA in diagnosis for hepatitis C was significantly higher than that of common HCV-RNA and HCV core antigen detection.Conclusion High-sensitive HCV-RNA is an important marker for the judgment of hepatitis C,and can be used for the diagnosis of hepatitis C treatment endpoint and disease monitoring.

关 键 词：丙型肝炎病毒 丙型肝炎病毒核糖核酸 高敏 丙型肝炎病毒核心抗原 诊断 

分 类 号：R446.9[医药卫生—诊断学]