脐带间充质干细胞对干燥综合征患者外周血的免疫调控研究  

Study on immune regulation of umbilical cord mesenchymal stem cells in peripheral blood of patients with Sjogren's syndrome

作　　者：王思博 吴涛 庞春艳[1] 王慧[1] WANG Sibo;WU Tao;PANG Chunyan;WANG Hui(The First Affiliated Hospital of Baotou Medical College,Baotou 014010,China)

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010

出　　处：《包头医学院学报》2024年第2期28-31,48,共5页Journal of Baotou Medical College

基　　金：内蒙古自治区自然科学基金项目(2018LH08068)。

摘　　要：目的:研究脐带间充质干细胞(UC-MSCs)对干燥综合征(SS)患者外周血的免疫调节作用。方法:取新生儿脐带组织分离并培养UC-MSCs,流式细胞术检测UC-MSCs的细胞表面标记;选取20例疾病活动期的SS患者,收集静脉血提取外周血单个核细胞(PBMC),将SS患者的PBMC与UC-MSCs共培养,CCK-8法检测细胞存活率,筛选最优共培养细胞比例进行后续实验;流式细胞术检测共培养后SS患者PBMC中淋巴细胞亚群的变化情况。结果:UC-MSCs可降低SS患者PBMC中的外周血CD3^(+)T细胞、CD3^(+)CD8^(+)T细胞以及CD19^(+)B细胞的比例,而CD16^(+)CD56^(+)NK细胞比例、CD3^(+)CD4^(+)T细胞比例、CD4^(+)/CD8^(+)无明显变化。结论:UC-MSCs可以调节SS患者的免疫功能,从而对SS有一定的治疗作用。Objective:To study the immunoregulatory effect of umbilical cord mesenchymal stem cells(UC-MSCs)on peripheral blood of patients with Sjogren's syndrome(SS).Methods:UC-MSCs were isolated and cultured from umbilical cord tissue of newborns,and the cell surface markers of UC-MSCs were detected by flow cytometry.Peripheral blood mononuclear cells(PBMCs)were collected from 20 SS patients in the active phase of the disease.Co-cultures of PBMCs from SS patients with UC-MSCs were established,and cell viability was assessed using the CCK-8 assay.The optimal ratio of co-cultured cells was determined for subsequent experiments.Flow cytometry was used to analyze the changes in lymphocyte subsets in SS patient PBMCs after co-culture.Results:UC-MSCs reduced the proportions of peripheral blood CD3^(+)T cells,CD3^(+)CD8^(+)T cells,and CD19^(+)B cells in PBMC of SS patients,while the proportions of CD16^(+)CD56^(+)NK cells,CD3^(+)CD4^(+)T cells,and CD4^(+)/CD8^(+)ratio were not significantly affected.Conclusion:UC-MSCs can modulate the immune function of SS patients,thereby exerting a certain therapeutic effect on SS.

关 键 词：干燥综合征 脐带间充质干细胞 T淋巴细胞亚群 免疫功能 

分 类 号：R593.2[医药卫生—内科学]