3种中药成分对氧化型低密度脂蛋白诱导人主动脉内皮细胞损伤的影响  

Effects of Three Traditional Chinese Medicine Ingredients on the Oxidized Low-Density Lipoprotein-Induced Human Aortic Endothelial Cells Injury

作　　者：楼彩霞[1,2] 王弋 代路路[1,2] 龙淑娴 黄小琼 LOU Caixia;WANG Yi;DAI Lulu;LONG Shuxian;HUANG Xiaoqiong(Guangdong Medical Laboratory Animal Center,Foshan,Guangdong,China 528248;Guangdong Provincial People's Hospital·Guangdong Academy of Medical Sciences,Guangzhou,Guangdong,China 510080)

机构地区：[1]广东省医学实验动物中心,广东佛山528248 [2]广东省人民医院·广东省医学科学院,广东广州510080

出　　处：《中国药业》2024年第4期39-42,共4页China Pharmaceuticals

基　　金：广东省中医药局面上科研项目[20191046]。

摘　　要：目的探讨3种中药成分(姜黄素、连翘苷、白藜芦醇)对氧化型低密度脂蛋白(Ox-LDL)诱导的人主动脉内皮细胞(HAEC)损伤的影响。方法实验设空白对照组(等体积基础培养液),溶剂对照组[等体积含二甲基亚砜(DMSO)的基础培养液],Ox-LDL组(20μg/mL Ox-LDL),姜黄素①②③④组(20μg/mL Ox-LDL+2.5μmol/L、5μmol/L、10μmol/L、20μmol/L姜黄素),连翘苷①②③④组(20μg/mL Ox-LDL+10μmol/L、20μmol/L、40μmol/L、80μmol/L连翘苷),白藜芦醇①②③组(20μg/mL Ox-LDL+20μmol/L、40μmol/L、80μmol/L白藜芦醇),采用四甲基偶氮噻唑盐(MTT)法检测细胞活性,并计算细胞增殖率;采用实时荧光定量聚合酶链式反应(qPCR)法检测细胞微管相关蛋白1轻链3(LC3)和环氧合酶2(COX-2)mRNA的表达水平。结果与Ox-LDL组比较,姜黄素、白藜芦醇各剂量组,连翘苷④组细胞增殖率均显著降低(P<0.01或P<0.05);姜黄素③组、连翘苷②③④组、白藜芦醇各剂量组细胞LC3 mRNA表达水平均显著升高,COX-2 mRNA表达水平均显著降低(P<0.01或P<0.05)。结论姜黄素、连翘苷和白藜芦醇可抑制Ox-LDL诱导下HAEC的增殖,可激发受损细胞自噬,抑制炎性因子COX-2表达。Objective To investigate the effects of three traditional Chinese medicine(TCM)ingredients(curcumin,phillyrin and resveratrol)on the oxidized low-density lipoprotein(Ox-LDL)-induced human aortic endothelial cells(HAECs)injury.Methods HAECs were divided into the blank control group(equal volume of basic culture medium),the solvent control group[equal volume of basic culture medium containing dimethyl sulfoxide(DMSO)],the Ox-LDL group(20μg/mL Ox-LDL),the curcumin groups①②③④(20μg/mL Ox-LDL+2.5μmol/L,5μmol/L,10μmol/L,20μmol/L curcumin),the phillyrin groups①②③④(20μg/mL Ox-LDL+10μmol/L,20μmol/L,40μmol/L,80μmol/L phillyrin),the resveratrol groups①②③(20μg/mL Ox-LDL+20μmol/L,40μmol/L,80μmol/L resveratrol).Cell activity was detected by the methyl thiazolyl tetrazolium(MTT)method,the cell proliferation rate was calculated,the expression levels of microtubule-associated protein 1 light-chain 3(LC3)and cyclooxygenase-2(COX-2)mRNA were detected by the real-time fluorescence quantitative polymerase chain reaction(qPCR).Results Compared with that in the Ox-LDL group,the cell proliferation rate in the phillyrin group④,all the curcumin groups and resveratrol groups significantly decreased(P<0.01 or P<0.05).Compared with those in the Ox-LDL group,the expression levels of LC3 mRNA in the curcumin group③,phillyrin groups②③④and all the resveratrol groups significantly increased,and the expression level of COX-2 mRNA significantly decreased(P<0.01 or P<0.05).Conclusion Curcumin,phillyrin and resveratrol can inhibit the proliferation of HAECs induced by Ox-LDL,stimulate the autophagy of injured cells,and inbibit the expression of inflammatory factor COX-2.

关 键 词：姜黄素 连翘苷 白藜芦醇 氧化型低密度脂蛋白 人主动脉内皮细胞 细胞自噬 炎性反应 

分 类 号：R965[医药卫生—药理学] R285[医药卫生—药学]