金水葶苓汤对肺癌移植瘤恶性胸腔积液模型小鼠的治疗作用及机制研究  

Effect and Mechanism of Jinshui Tingling Decoction(金水葶苓汤)on the Mouse Model of Pleural Effusion in Lung Cancer Transplant Tumors

作　　者：赵林涛[1] 缑娇 王松海 杨宝钢 郭弈飞 宋延平[1] 杨晨光 ZHAO Lintao;GOU Jiao;WANG Songhai;YANG Baogang;GUO Yifei;SONG Yanping;YANG Chenguang(Shaanxi Institute of Traditional Chinese Medicine,Xi`an Shaanxi 710003,China;Shaanxi Hospital of Traditional Chinese Medicine,Xi'anShaanxi710003,China;Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046,China)

机构地区：[1]陕西省中医药研究院,陕西西安710003 [2]陕西省中医医院,陕西西安710003 [3]陕西中医药大学,陕西咸阳712046

出　　处：《中医药导报》2024年第1期26-30,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省自然科学基础研究计划(2021JM-565)。

摘　　要：目的:通过复制Lewis肺癌移植瘤胸腔积液小鼠模型,考察金水葶苓汤对模型小鼠胸腔积液的抑制作用。方法:通过胸腔注射Lewis细胞复制C57小鼠胸腔积液肺癌模型,观察金水葶苓汤及金水葶苓汤协同顺铂注射液对模型小鼠胸腔积液体积、胸壁转移瘤数量和转移瘤质量、生存时间的影响;采用Western blotting法检测壁层胸膜AQP1和胸腔积液中VEGF蛋白表达的水平,评价金水葶苓汤对肺癌移植瘤胸腔积液小鼠的治疗作用和机制。结果:与模型组比较,各给药组小鼠体质量增长明显升高,胸腔积液体积、胸壁转移瘤数量、转移瘤质量明显降低,小鼠胸腔积液中VEGF及胸膜中AQP1蛋白表达明显低于模型组,差异均有统计学意义(P<0.05或P<0.01),且金水葶苓汤+顺铂组比单用金水葶苓汤或顺铂注射液效果更好,差异有统计学意义(P<0.05)。结论:金水葶苓汤可以抑制恶性胸腔积液模型小鼠胸腔积液生成,改善小鼠体质量,延长小鼠生存时间,降低胸壁肿瘤转移数量和抑制肿瘤生长,降低胸腔积液中VEGF及胸膜中AQP1蛋白表达水平,且金水葶苓汤协同顺铂注射液优于金水葶苓汤或顺铂注射液单用效果。Objective:To investigate the inhibitory effect of Jinshui Tingling decoction on pleural effusion by replicating the mouse model of LEWIS lung cancer transplant tumor pleural effusion.Methods:The pleural effusion lung cancer model of C57 mice was replicated by LEWIS cells chest injection.The effect of Jinshui Tingling decoction and Jinshui Tingling decoction combined with cisplatin injection on the volume of pleural effusion,number and quality of metastatic tumors in the chest wall metastases,and survival time was observed.Western blotting method was used to detect the expression levels of AQP1 protein in parietal pleural and VEGF protein in pleural effusion,to evaluate the therapeutic effect and mechanism of Jinshui Tingling decoction on pleural effusion mice with lung cancer transplant tumor.Results:Compared with the model group,the weight gain of mice was significantly increased in each drug delivery group,and the volume of pleural fluid,the number of metastases of the chest wall and the weight of metastases were significantly reduced in each drug delivery group.The drug delivery groups showed lower expression of VEGF and AQP1 protein than model group(P<0.05 or P<0.01),and Jinshui Tingling decoction+cisplatin injection group showed better effect than Jinshui Tingling decoction or cisplatin injection(P<0.05).Conclusion:Jinshui Tingling decoction can inhibit the generation of pleural effusion in malignant pleural effusion model mice,improve body weight,prolong survival time,reduce the number of chest wall tumor metastases,inhibit tumor growth,and decrease the expression levels of VEGF in pleural effusion and AQP1 protein in pleura.Moreover,Jinshui Tingling decoction combined with cisplatin injection shows better effect than Jinshui Tingling decoction and cisplatin injection alone.

关 键 词：恶性胸腔积液 肺癌 金水葶苓汤 顺铂注射液 血小板内皮生长因子 水道蛋白1 小鼠 

分 类 号：R285.5[医药卫生—中药学]