异丙肾上腺素诱导小鼠急性心肌梗死模型制备方法研究  

Preparation method of acute myocardial infarction model induced by isoproterenol in mice

作　　者：钱铭 程龙 熊媛 杨慧 吉利 QIAN Ming;CHENG Long;XIONG Yuan;YANG Hui;JI Li(Department of Pharmacy,Affiliated Drum Tower Hospital of Medical School of Nanjing University,Nanjing,Jiangsu,210008;Drum Tower Clinical Medical College,Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu,210008;School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing,Jiangsu,210008;Department of Geriatrics,Affiliated Drum Tower Hospital of Medical School of Nanjing University,Nanjing,Jiangsu,210008)

机构地区：[1]南京大学医学院附属鼓楼医院药学部,江苏南京210008 [2]南京中医药大学鼓楼临床医学院,江苏南京210008 [3]中国药科大学基础医学与临床药学学院,江苏南京210008 [4]南京大学医学院附属鼓楼医院老年医学科,江苏南京210008

出　　处：《实用临床医药杂志》2023年第23期6-11,共6页Journal of Clinical Medicine in Practice

基　　金：2022年国家自然科学基金青年项目(82204720)。

摘　　要：目的探讨异丙肾上腺素给药方式对小鼠心肌损伤的影响,建立小鼠急性心肌梗死(AMI)模型。方法小鼠皮下注射异丙肾上腺素(150 mg/kg),于不同时点取小鼠血样,检测血清中AMI相关指标[肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T(cTnT)、乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、白介素6(IL-6)、降钙素原(PCT)和C反应蛋白(CRP)]的含量变化;通过苏木素-伊红染色观察小鼠心脏组织的病理改变。结果造模后,血清中CK、CK-MB、LDH、cTnT、ALT、AST水平升高(P<0.05或P<0.01);2次连续给予异丙肾上腺素,小鼠血清中AMI相关指标升高,且相关指标在给药后16 h达到最高浓度(P<0.05或P<0.01)。苏木素-伊红染色结果显示,肌组织发生病理学改变。结论皮下注射异丙肾上腺素可诱导小鼠AMI,其中连续2次注射后16 h心肌损伤最为严重,这为AMI的临床治疗药物研究和创新药物开发奠定基础。Objective To investigate the effects of isoproterenol administration on myocardial injury and establish acute myocardial infarction(AMI)model in mice.Methods Mice were injected subcutaneously with isoproterenol(150 mg/kg),and blood samples were taken at different time points.The contents of AMI related indexes[creatine kinase(CK),creatine kinase isoenzyme(CK-MB),cardiac troponin T(cTnT),lactate dehydrogenase(LDH),alanine aminotransferase(ALT),aspartate aminotransferase(AST),interleukin 6(IL-6),procalcitonin(PCT)and C-reactive protein(CRP)]in serum were detected.The pathological changes of cardiac tissue were observed by hematoxylin-eosin staining.Results The serum levels of CK,CK-MB,LDH,cTnT,ALT and AST were significantly increased after isoproterenol model(P<0.05,P<0.01);after two consecutive administrations of isoproterenol,the serum AMI-related indexes increased significantly,and the relevant indexes reached the highest concentration 16 h after administration(P<0.05,P<0.01).Hematoxylin-eosin staining results showed that pathological changes occurred in myocardial tissue.Conclusion Subcutaneous injection of isoproterenol induces AMI injury.The AMI injury at 16 h after two consecutive injections is the most serious,which lays a foundation for clinical drug research and innovative drug development of AMI.

关 键 词：急性心肌梗死 异丙肾上腺素 肌酸激酶 肌酸激酶同工酶 心肌肌钙蛋白T 乳酸脱氢酶 

分 类 号：R446.1[医药卫生—诊断学] R543.3[医药卫生—临床医学] R542.2