清热生肌膏介导NLRP3对放射性皮炎大鼠MPO、Hyp表达的影响  

Qingreshengji ointment mediates the effect of NLRP3 on MPO and Hyp expression in rats with radiation dermatitis

作　　者：杨文博 孙云川[1] 杨洪娟 杜强 徐旭英 YANG Wenbo;SUN Yunchuan;YANG Hongjuan;DU Qiang;XU Xuying(Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061000,Hebei,China;Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100000,China)

机构地区：[1]河北省沧州中西医结合医院,河北沧州061000 [2]首都医科大学附属北京中医院,北京100000

出　　处：《西部医学》2024年第1期47-51,共5页Medical Journal of West China

基　　金：国家自然科学基金委员会资助项目(81673975);河北省中医药管理局中医药科研计划项目(2020512)。

摘　　要：目的 清热生肌膏介导NLRP3炎性小体对放射性皮炎大鼠皮肤损伤修复及MPO、Hyp表达水平的影响。方法 选取SD健康大鼠90只,80只随机分为正常组、模型组(放射性皮炎大鼠模型)、治疗组(模型+清热生肌膏)、对照组(模型+复方茶多酚软膏),每组各20只。另设抑制剂组(NLRP3抑制剂)10只。通过创面愈合率衡量创面愈合情况。透射电镜观察创面细胞超微结构。HE染色观察病理学变化。分光光度比色法测定MPO活性。免疫印迹检测NLRP3、Caspase-1蛋白表达。结果 各组在治疗后第1 d时的创面愈合无差异(P>0.05)。模型组大鼠在治疗3~21 d时的创面愈合均低于治疗组及对照组(P<0.05)。治疗组与对照组大鼠治疗治疗3~21 d各时间点创面愈合比较无差异(P>0.05)。与正常组相比,模型组大鼠MPO表达升高、Hyp表达降低(P<0.05)。与模型组相比,治疗组大鼠MPO表达降低、Hyp表达升高(P<0.05)。治疗组与对照组MPO及Hyp表达对比无差异(P>0.05)。与正常组相比,模型组大鼠NLRP3、Caspase-1表达升高(P<0.05)。与模型组相比,治疗组大鼠NLRP3、Caspase-1蛋白表达降低(P<0.05)。治疗组与抑制剂组大鼠NLRP3、Caspase-1蛋白表达对比无差异(P>0.05)。结论 清热生肌膏可抑制MPO水平并促进Hyp表达,对放射性皮炎大鼠皮肤损伤修复有积极促进作用,其作用机制可能与介导NLRP3炎性小体有关。Objective To investigate the effects of Qingreshengji ointment on NLRP3 inflammasome on skin injury repair and expression levels of MPO and Hyp in rats with radioactive dermatitis.Methods Ninety healthy SD rats were selected and provided by Changsha Tianqin Company.Eighty rats were randomly divided into normal group,model group(radiation dermatitis rat model),treatment group(model+Qingreshengji ointment) and control group(model+compound tea polyphenol ointment),with 20 rats in each group.Another inhibitor group(NLRP3 inhibitor) consisted of 10 subjects.Wound healing was measured by wound healing rate.The ultrastructure of wound tissue was observed by transmission electron microscope.Histopathological changes of wound skin were observed by HE staining.The activity of MPO was determined by spectrophotometric colorimetry.NLRP3 and Caspase-1 were detected by western blotting.Results There was no difference in wound healing among all groups on day 1 after treatment(P>0.05).The wound healing of model group was lower than that of treatment group and control group at 3-21 d(P<0.05).There was no difference in wound healing between the treatment group and the control group at each time point of 3-21 d(P>0.05).Compared with normal group,MPO expression was increased and Hyp expression was decreased in model group(P<0.05).Compared with model group,MPO expression was decreased and Hyp expression was increased in treatment group(P<0.05).There were no differences in MPO and Hyp expression between treatment group and control group(P>0.05).Compared with normal group,the expressions of NLRP3 and Caspase-1 in model group were increased(P<0.05).Compared with model group,NLRP3 and Caspase-1 protein expressions in treatment group were decreased(P<0.05).There were no differences in NLRP3 and Caspase-1 protein expressions between treatment group and inhibitor group(P>0.05).Conclusion Qingre Shengji ointment can inhibit the level of MPO and promote the expression of Hyp,and has a positive effect on the repair of skin damage in rats with r

关 键 词：清热生肌膏 NLRP3炎性小体 放射性皮炎大鼠 皮肤损伤修复 MPO、Hyp表达水平 

分 类 号：R751[医药卫生—皮肤病学与性病学]