膝关节骨性关节炎患者软骨组织中α激酶1的表达变化及意义  

Changes and significance ofαkinase 1 expression in cartilage tisssueof patients with knee osteoarthritis

作　　者：耿庆贺 兰俊赟 王申 衡科[5] 宋星辰 聂培如 张环宇 刘艳红 郭依龙 GENG Qinghe;LAN Junyun;WANG Shen;HENG Ke;SONG Xingchen;NIE Peiru;ZHANG Huanyu;LIU Yanhong;GUO Yilong(Central Laboratory,Pizhou Hospital Affiliated to Xuzhou Medical University,Xuzhou,Jiangsu 221300,China;不详)

机构地区：[1]徐州医科大学附属邳州医院中心实验室徐州医科大学骨质疏松症临床研究重点实验室,江苏邳州221300 [2]徐州医科大学附属邳州医院骨科,江苏邳州221300 [3]青海省兴海县人民医院外科,青海海南州813300 [4]北京大学医学部,北京100000 [5]南京医科大学附属常州二院骨科,江苏常州213000 [6]南京医科大学康达学院,江苏连云港222000

出　　处：《中国临床研究》2024年第1期85-88,共4页Chinese Journal of Clinical Research

基　　金：中国国家博士后基金委面上项目(2020M681739);江苏省卫健委医学科研项目(LKM2022048,Z2021046);徐州市科技局科研项目(KC22273);徐州医科大学江苏省重点实验室开放课题(XZSYSKF2020004,XZSYSKF2020033)。

摘　　要：目的 探讨α激酶家族成员之一的α激酶1(ALPK1)在膝关节骨关节炎(OA)的软骨组织中的表达变化及意义。方法 回顾性选择2018年7月至2022年10月于徐州医科大学附属邳州医院因膝关节OA住院手术的25例患者,收集患者膝关节软骨组织标本。另选择该院生物样品库中10例因创伤行“下肢截肢术”患者的胫骨平台软骨作为对照。采用免疫组化染色、Western blotting、实时定量PCR(RT-qPCR)检测ALPK1在软骨中的表达变化。结果 免疫组化染色、Western blotting及RT-qPCR检测结果显示,与对照组相比,膝关节OA患者软骨中ALPK1 mRNA表达水平(2.126±0.930 vs 0.995±0.049,t=4.112,P<0.01)、蛋白表达水平(1.880±0.722 vs 1.025±0.062,t=3.706,P<0.01)和免疫组化阳性细胞率(P<0.01)均显著增高,差异有统计学意义。结论 膝关节OA患者软骨中ALPK1呈现高表达,ALPK1表达增加可能是关节软骨炎性增加、软骨破坏的原因之一。ALPK1有望作为膝关节OA的诊断标志物和治疗靶标。Objective To investigate the expression changes and significance ofαkinase 1(ALPK1),a member of theαkinase family,in cartilage tissue of knee osteoarthritis(OA).Methods Twenty-five patients who underwent knee OA surgery in Pizhou Hospital affiliated to Xuzhou Medical University from July 2018 to October 2022 were retrospectively selected,and cartilage tissue samples of the patients knee joints were collected.In addition,the tibial plateau cartilage of 10 patients who underwent lower limb amputation due to trauma in the biological sample of the hospital was selected as the control.Immunohistochemical(IHC)staining,Western blotting and real-time quantitative PCR(RT-qPCR)were used to detect the expression of ALPK1 in cartilage.Results The results of IHC staining,Western blotting and RT-qPCR showed that compared with the control group,the mRNA expression level of ALPK1(2.126±0.930 vs 0.995±0.049,t=4.112,P<0.01),protein expression level(1.880±0.722 vs 1.025±0.062,t=3.706,P<0.01)and IHC-positive cell rate(P<0.01)in the cartilage of knee OA patients were significantly increased.Conclusion The high expression of ALPK1 in cartilage of patients with kee OA may be one of the reasons for the increase of inflammation and destruction of articular cartilage.ALPK1 is expected to be a diagnostic marker and therapeutic target of kee OA.

关 键 词：骨关节炎 软骨 α激酶1 标志物 靶标 

分 类 号：R684.3[医药卫生—骨科学]