F-53B胁迫下污水厌氧处理系统及微生物群落的响应  

Response of wastewater anaerobic treatment system and microbial community under F-53B stress

作　　者：郭雨婷 刘亚军 邓觅[2] 涂勋 吴虎彬 李昆[1] 李荣富 吴永明[1,2] GUO Yu-ting;LIU Ya-jun;DENG Mi;TU Xun;WU Hu-bin;LI Kun;LI Rong-fu;WU Yong-ming(Key Laboratory of Poyang Lake Environment and Resource Utilization,Ministry of Education,School of Resources&Environment,Nanchang University,Nanchang 330031,China;Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang 330096,China)

机构地区：[1]南昌大学资源与环境学院,鄱阳湖环境与资源利用教育部重点实验室,江西南昌330031 [2]江西省科学院微生物研究所,江西南昌330096

出　　处：《中国环境科学》2024年第2期721-729,共9页China Environmental Science

基　　金：江西省科技计划项目(20232BCJ22047,20212BCD42014,20213AAG01012);江西省科学院省级科技计划项目包干制试点示范项目(2021YSBG10003,2023YRCS001,2023YSBG22012,2021YSBG22030);江西省自然科学基金资助项目(20212BAB213041)。

摘　　要：通过构建厌氧反应体系,评估低浓度(1mg/L)和高浓度(10mg/L)氯化多氟醚磺酸盐(F-53B)胁迫下,厌氧处理系统出水水质、污泥性状和细菌群落的响应特征.结果表明,在F-53B的胁迫下,COD、氮(TN、NH_(4)^(+)-N)和磷(TP、PO_(4)^(3-)-P)在出水中的含量会升高,而污泥相对生物量(MLVSS/MLSS)则出现下降,且F-53B的浓度越高影响越大.高通量测序分析发现,相较于对照组(0mg/L F-53B),仅高浓度F-53B胁迫下细菌群落的多样性(ACE、Chao1和PD指数)出现了显著下降.同时,研究还发现,随着高浓度F-53B暴露时间的延长,厚壁菌门的相对丰度逐渐增大,而变形菌门、拟杆菌门和硝化螺旋菌门的相对丰度逐渐降低.此外,基于FAPROTAX功能预测分析发现,F-53B胁迫下微生物表现出更低的氮(N)代谢潜力和更高的化能异养和发酵能力.冗余分析(RDA)表明,厚壁菌门主要受F-53B的正向影响,并与TN和NH_(4)^(+)-N呈正相关;而变形菌门受F-53B的负向影响,并与COD、TP和PO_(4)^(3-)-P呈正相关.揭示了新型氟化物—F-53B对污水厌氧处理系统的胁迫机制.By constructing an anaerobic reaction system,evaluate the response characteristics of effluent water quality,sludge properties,and bacterial communities under low concentration(1mg/L)and high concentration(10mg/L)of chlorinated polyfluorinated ether sulfonic acids(F-53B)stress.The results showed that under F-53B stress,the contents of COD,nitrogen(TN,NH4+-N),and phosphorus(TP,PO_(4)^(3-)-P)in the effluent were elevated,while the relative biomass of sludge(MLVSS/MLSS)showed a decrease,and the higher the concentration of F-53B,the greater the effect.High-throughput sequencing analysis revealed that only the diversity of bacterial communities(ACE,Chao1,and PD_whole_tree indices)significantly decreased under high concentration F-53B stress compared to the control group(0mg/L F-53B).Additionally,the study found that with prolonged exposure to high concentration F-53B,the relative abundance of Firmicutes phylum gradually increased,while the relative abundance of Proteobacteria,Bacteroidota and Nitrospirota decreased.Furthermore,based on FAPROTAX functional prediction analysis,microorganisms exhibited lower nitrogen(N)metabolism potential and higher chemoheterotrophic and fermentative capabilities under F-53B stress.Redundancy analysis(RDA)indicated that the Firmicutes was mainly positively influenced by F-53B and positively correlated with TN and NH4+-N,while the Proteobacteria was negatively influenced by F-53B and positively correlated with COD,TP,and PO_(4)^(3-)-P.Revealed the stress mechanism of a novel fluoride compound,F-53B,on wastewater anaerobic treatment systems.

关 键 词：氯化多氟醚磺酸盐(F-53B) 污水厌氧处理 微生物群落 功能预测 

分 类 号：X703[环境科学与工程—环境工程]