基于UHPLC-Q-TOF-MS/MS技术的中药荆芥穗化学成分表征与鉴定  

Characterization and identification of chemical constituents from Schizonepetae Spica based on UHPLC-Q-TOF-MS/MS technique

作　　者：赵毅萌 张礼欣 杨舒昀 王泽琨 李长印 束雅春 ZHAO Yi-meng;ZHANG Li-xin;YANG Shu-yun;WANG Ze-kun;LI Chang-yin;SHU Ya-chun(Department of Pharmacy,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Department of Clinical Pharmacology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;Traditional Chinese Medicine Processing Technology Inheritance Base,Jiangsu Provincial Hospital of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院药学部,江苏南京210029 [2]南京中医药大学附属医院江苏省中医院临床药理实验室,江苏南京210029 [3]江苏省中医院全国中药炮制技术传承基地,江苏南京210029

出　　处：《中国中药杂志》2024年第2期420-430,共11页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中药炮制技术传承创新项目(GZY-KJS-2022-053)。

摘　　要：应用超高效液相色谱-四极杆飞行时间质谱联用技术(UHPLC-Q-TOF-MS/MS)对荆芥穗中的化学成分进行定性分析。采用Agilent poroshell 120 SB-C_(18)色谱柱(3.0 mm×100 mm,2.7μm),以0.1%甲酸水(A)-乙腈(B)溶液为流动相进行梯度洗脱,流速0.4 mL·min^(-1),柱温45℃,电喷雾离子源,在正、负离子模式下扫描采集数据,通过对照品比对及PeakView软件分析鉴定化合物。从荆芥穗中共鉴定出97个化合物,其中黄酮类化合物28个,酚酸类化合物23个,脂肪酸类化合物23个,萜类化合物15个以及其他类化合物8个。该研究建立的UHPLC-Q-TOF-MS/MS能快速、准确、较全面地鉴定荆芥穗药材的化学成分,为荆芥穗药效物质基础研究提供参考依据。The chemical constituents of Schizonepetae Spica were qualitatively analyzed by UHPLC-Q-TOF-MS/MS.An Agilent poroshell 120 SB-C_(18) column(3.0 mm×100 mm,2.7 μm) was used for gradient elution with 0.1% formic acid water(A)-acetonitrile(B) solution as mobile phase at the flow rate of 0.4 mL·min~(-1) and column temperature of 45 ℃.The data were collected by scanning in positive and negative ion modes,and the compounds were identified by comparison of reference materials and PeakView software.Ninety-seven compounds were identified from Schizonepetae Spica,including 28 flavonoids,23 phenolic acids,23 fatty acids,15 terpenoids,and 8 other compounds.The UHPLC-Q-TOF-MS/MS method established in this study can identify the chemical components of Schizonepetae Spica rapidly,accurately,and comprehensively,and provide a basis for the basic study of pharmacodynamic substances of Schizonepetae Spica.

关 键 词：荆芥穗 超高效液相色谱-四极杆飞行时间质谱联用技术 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]