外翻肠囊法研究五味清浊散的肠吸收成分及其吸收特征  

Intestinal absorption components and absorption characteristics of Wuwei Qingzhuo San by everted intestinal sac models

作　　者：廖翠平 董世奇 刘云璐 额尼日勒 高雅 樊慧蓉[2] 蔡秋杰 代金刚[1] 图雅[5] LIAO Cui-ping;DONG Shi-qi;LIU Yun-lu;Enirile;GAO Ya;FAN Hui-rong;CAI Qiu-jie;DAI Jin-gang;TU Ya(Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Tianjin 300192,China;College of Mongolian Medicine and Pharmacy,Inner Mongolia Minzu University,Tongliao 028000,China;Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;Chinese Medicine Research and Development Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国医学科学院放射医学研究所,天津300192 [3]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [4]中国中医科学院临床基础研究所,北京100700 [5]中国中医科学院中医药研究发展中心,北京100700

出　　处：《中国中药杂志》2024年第2期509-517,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(82274212);中国中医科学院医学实验中心协同创新团队项目(XTCX2021004)。

摘　　要：研究五味清浊散在不同肠段的吸收情况,以及其生物碱类成分胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁的吸收特征。通过构建大鼠肠外翻模型,应用UPLC-Q-TOF-MS技术测定五味清浊散在不同肠段透过的化学成分,以及采用UPLC-Q-TRAP-MS技术测定肠吸收液中胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁的含量,计算其吸收参数,并分析其在大鼠不同肠段、不同时间的吸收特征。实验结果显示,在建立的UPLC-Q-TOF-MS条件下,在空肠、回肠、十二指肠和结肠段的肠囊液中分别检测到27、27、8、6个吸收成分;且发现五味清浊散中胡椒碱、胡椒新碱、荜茇宁和二氢荜茇宁在各肠段均为线性吸收,r均达到0.9以上,各肠段的吸收量胡椒碱>胡椒新碱>二氢荜茇宁>荜茇宁,但各成分在各个肠段的吸收速率及其机制不尽相同,提示在不同肠段五味清浊散提取液各成分的吸收具有选择性,并不是简单的半透膜渗透过程。This study investigated the absorption profile of Wuwei Qingzhuo San in different intestinal segments and the absorption characteristics of its alkaloids(piperine,piperanine,piperlonguminine,and dihydropiperlonguminine).The everted gut sac model was established,and the chemical components of Wuwei Qingzhuo San in different intestinal segments were detected by UPLC-Q-TOF-MS.The content of piperine,piperanine,piperlonguminine,and dihydropiperlonguminine in intestinal absorption fluid was determined by UPLC-Q-TRAP-MS and the absorption parameters were calculated.The absorption characteristics in different intestinal segments at different time were analyzed.As a result,27,27,8,and 6 absorbent components from Wuwei Qingzhuo San were detected in the intestinal cyst fluid of jejunum,ileum,duodenum,and colon by UPLC-Q-TOF-MS technology,respectively.It was also found that piperine,piperanine,piperlonguminine,and dihydropiperlonguminine from Wuwei Qingzhuo San showed linear absorption in various intestinal segments,with r values exceeding 0.9.In terms of absorption content,the components were ranked as piperine>piperanine>dihydropiperlonguminine>piperlonguminine in various intestinal segments,but the absorption rate and mechanism of each component varied.The results demonstrate that the absorption of the components of Wuwei Qingzhuo San in different intestinal segments is selective and is not a simple semi-permeable membrane permeation process.

关 键 词：五味清浊散 胡椒碱 胡椒新碱 荜茇宁 二氢荜茇宁 肠外翻模型 

分 类 号：R285[医药卫生—中药学]