动物细胞培养用片状载体特性分析  

Characteristic analysis of fibracel carriers for animal cell culture

作　　者：马春英 赵蕾 王子凡 侯刚 马花 马忠仁[1,3] 王家敏[1,3,4] MA Chunying;ZHAO Lei;WANG Zifan;HOU Gang;MA Hua;MA Zhongren;WANG Jiamin(Gansu Tech Innovation Center of Animal Cell,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,Gansu Province,China;不详)

机构地区：[1]西北民族大学生物医学研究中心甘肃省动物细胞技术创新中心,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,甘肃兰州730030 [4]甘肃省生物工程材料工程研究中心,甘肃兰州730010

出　　处：《中国生物制品学杂志》2024年第1期26-31,共6页Chinese Journal of Biologicals

基　　金：甘肃省科技计划(18JR2JA004,22JR11RA239);西北民族大学中央高校基本科研业务费专项资金(31920220021,31920220022)。

摘　　要：目的探讨不同片状载体的表面特性及对不同细胞的培养效果。方法选择3种片状载体(A、B、C),对其材料进行化学元素组成分析,对经0.1 mol/L NaOH溶液预处理前后的载体进行接触角检测。通过测定Vero、MDCK和MRC-5细胞在3种载体上的贴附效果和葡萄糖消耗量,并利用荧光染色法观察细胞生长状态,比较3种载体的细胞贴附率和培养效果。结果3种载体均主要由C、H、O 3种元素组成,且含有少量的N和S元素。预处理前,载体B的接触角为0°,显著低于载体A[(109±3.13)°]和C[(121±6.82)°](F均为709.1,P均<0.0001),亲水性较强,载体A和C亲水性较差;预处理后,3种载体表面的接触角均为0°,差异无统计学意义(F=0.0694,P>0.05),均亲水。培养3 h内,3种载体的细胞贴附率均在80%以上,细胞在载体纤维上均致密,长势均匀,葡萄糖消耗量曲线均趋于“S”型,细胞连续培养效果良好。葡萄糖消耗总量载体A与C基本一致,载体B低于A和C。结论对3种片状载体的化学元素组成和亲疏水特性关系进行了分析,并对Vero、MDCK和MRC-53种细胞的贴附率及培养效果进行了评价,为片状载体的后续研究及生产应用提供了参考。Objective To investigate the surface properties of different fibracel carriers and their culture effects on different cells.Methods Three fibracel carriers(A,B,C)were selected to analyze the chemical element composition of their materials,and the contact angles of the carriers before and after pretreatment with 0.1 mol/L NaOH solution were tested.By measuring the adhesion effect and glucose consumption of Vero,MDCK and MRC-5 cells on the carrier,and observing the cell growth state by fluorescent staining,the cell adhesion efficiency and culture effect of the three carriers were compared and analyzed.Results The three carriers were mainly composed of C,H,O,and contained a small amount of N and S elements.Before pretreatment,the contact angle of carrier B was 0°,which was significantly lower than that of A[(109 ± 3.13)°]and C[(121 ± 6.82)°](each F = 709.1,each P < 0.000 1),and the hydrophilicity was stronger.Carriers A and C had poor hydrophilicity.After pretreatment,the contact angles of the surfaces of the three carriers A,B,and C were all 0°,with no significant difference(F = 0.069 4,P > 0.05),all of which were hydrophilic.The adherence rates of the three types of carriers within 3 h of cell culture were all above 80%.The cells were dense and evenly grown on the carrier fibers,the glucose consumption curves tended to“S”type,and the continuous cell culture effect was good.The total glucose consumption of carrier A and carrier C was basically the same,and carrier B was lower than carrier A and carrier C.Conclusion The chemical element composition and the relationship between the hydrophilic and hydrophobic properties of the three fibracel carriers were analyzed,and the adhesion rate and culture effect of Vero,MDCK and MRC-5 cells were evaluated,which provide reference for the subsequent research and production application of fibracel carriers.

关 键 词：片状载体 元素分析 细胞培养 

分 类 号：Q813.1[生物学—生物工程]