HOXB4基因表达水平在骨髓增生异常综合征中的临床意义  

Clinical significance of HOXB4 gene expression levels in myelodysplastic syndromes

作　　者：王怡晨 严燕雯 宋美慧 薛祥俊 周文广 李玉权 齐玲 李光华 曾祥宗 WANG Yichen;YAN Yanwen;SONG Meihui;XUE Xiangjun;ZHOU Wenguang;LI Yuquan;QI Ling;LI Guanghua;ZENG Xiangzong(College of Basic Medicine,Dali University,Dali 671000,China;Institute of Digestive Disease,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital,Qingyuan 511518,China;12)

机构地区：[1]大理大学基础医学院,云南大理671000 [2]广州医科大学附属第六医院,清远市人民医院消化病研究所,广东清远511518 [3]广州医科大学附属第六医院,清远市人民医院血液病科,广东清远511518

出　　处：《实用医学杂志》2024年第3期321-325,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82203351);广东省中医药管理局基金项目(编号:20211458);清远市人民医院博士后人才项目(编号:15001019001141);清远市人民医院临床研究专项基金项目(编号:QYRYCRC2023011);清远市人民医院研究生基金项目(编号:20220327)。

摘　　要：目的探讨HOXB4基因在骨髓增生异常综合征(myelodysplastic syndromes,MDS)患者中的表达情况及其在疾病进展中的临床意义。方法利用实时荧光定量PCR(RT-qPCR)法检测骨髓单个核细胞HOXB4基因的mRNA表达水平,对比49例MDS患者(MDS组)和35例非恶性血液系统疾病患者(C组)的HOXB4基因的表达差异,并探讨HOXB4的表达水平与MDS疾病特征及临床预后之间的关系。结果MDS组HOXB4基因的表达水平较C组高(P<0.05)。根据HOXB4的中位表达水平分为高表达组和低表达组,高表达组患者初诊时白细胞水平低于低表达组,而伴原始细胞增多的MDS亚型、不良预后分型及白血病转化的患者比例高于低表达组(P<0.05)。结论HOXB4基因的表达水平与MDS患者向急性白血病转化有一定关联性。Objective To investigate the expression of HOXB4 gene in patients with myelodysplastic syn⁃dromes(MDS)and its clinical significance in disease progression.Methods mRNA expression of HOXB4 gene in bone marrow mononuclear cells was detected by real⁃time fluorescence quantitative PCR(RT⁃qPCR),and the difference in HOXB4 expression was compared between 49 patients with MDS(MDS group)and 35 patients without MDS(group C).The relationship of mRNA expression of HOXB4 with disease characteristics and clinical prognosis was explored in MDS patients.Results mRNA expression level of HOXB4 gene was higher in MDS group than that in group C(P<0.05).The patients were divided into a high⁃and a low⁃expression group according to the median expression level of HOXB4.Leukocyte count was lower in the high⁃expression group in the low⁃expression group at the time of initial diagnosis.The proportion of patients with subtypes of primitive cellular hyperplasia,poor prognostic staging and leukemic transformation was higher in the high⁃expression group than in the low⁃expression group.Conclusions mRNA expression level of HOXB4 gene has certain relation with AML transformation in MDS patients.

关 键 词：骨髓增生异常综合征 HOXB4 预后 白血病转化 

分 类 号：R551[医药卫生—血液循环系统疾病]