葛根素对3T3-L1脂肪细胞胰岛素抵抗的影响及机制研究  

Effect of puerarin on insulin resistance in 3T3-L1 adipocytes and its mechanism

作　　者：白继昌 谈力欣[2] 刘赞朝[3] 杨洋[1] 朱亚军[4] BAI Jichang;TAN Lixin;LIU Zanchao;YANG Yang;ZHU Yajun(Department of Endocrinology,Shijiazhuang 050000,China;Department of Diabetes,Shijiazhuang 050000,China;Key Laboratory,the Second Hospital of Shijiazhuang,Shijiazhuang 050000,China;Department of Endocrinology,Hebei General Hospital,Shijiazhuang 050051,China)

机构地区：[1]河北石家庄市第二医院内分泌科,石家庄050000 [2]河北石家庄市第二医院糖尿病五科,石家庄050000 [3]河北石家庄市第二医院重点实验室,石家庄050000 [4]河北省人民医院内分泌科,石家庄050051

出　　处：《广西医科大学学报》2024年第1期40-45,共6页Journal of Guangxi Medical University

基　　金：河北省2018年度医学科学研究重点课题计划项目(No.20180352)。

摘　　要：目的:探讨葛根素对3T3-L1脂肪细胞胰岛素抵抗(IR)的影响及可能的作用机制。方法:将3T3-L1脂肪细胞分为7组,即对照组(control组)、葛根素3μmol/L组、葛根素10μmol/L组、葛根素30μmol/L组、葛根素100μmol/L组、葛根素300μmol/L组和阳性对照组(罗格列酮10μmol/L组,RGZ组),每组6孔。采用MTT法检测细胞增殖,油红O染色法检测细胞分化。利用地塞米松诱导建立3T3-L1脂肪细胞IR模型,并给予不同浓度的葛根素进行干预,测定葡萄糖利用情况,利用细胞转染过表达TLR2(命名为Pue+oe-TLR2组);蛋白质免疫印迹法(western blotting)测定TLR2蛋白水平;酶联免疫吸附试验测定干扰素-γ(IFN-γ)水平。结果:与control组相比,葛根素各剂量组3T3-L1脂肪细胞的增殖率均无显著变化(P>0.05)。与control组相比,葛根素各剂量组3T3-L1脂肪细胞的分化明显增加(P<0.05)。与control组相比,葛根素各剂量组IR 3T3-L1脂肪细胞的葡萄糖消耗量显著提高(P<0.05);葛根素各剂量组IR 3T3-L1脂肪细胞中TLR2表达量、IFN-γ分泌量降低,GLUT4和PPARγ的水平升高(P<0.05)。Pue+oe-TLR2组TLR2的相对表达量及IFN-γ分泌量显著高于Pue+oe-NC组,PPARγ、GLUT4的相对表达量显著低于Pue+oe-NC组(P<0.01)。结论:葛根素可提高IR 3T3-L1脂肪细胞葡萄糖消耗,缓解IR,其机制可能与抑制TLR2表达进而降低IFN-γ分泌有关。Objective:To investigate the effect of puerarin on insulin resistance(IR)in 3T3-L1 adipocytes and its possible mechanism.Methods:3T3-L1 adipocytes were divided into 7 groups,namely control group,3μmol/L puerarin group,10μmol/L puerarin group,30μmol/L puerarin group,100μmol/L puerarin group,300μmol/L puerarin group,and positive control group(rosiglitazone 10μmol/L group,RGZ group),with 6 Wells in each group.Cell proliferation was detected by MTT assay,and adipocyte differentiation was detected by oil red O staining.The IR model of 3T3-L1 adipocytes was established by dexamethasone induction,and the glucose utilization was measured after different concentrations of puerarin treatment.Cells were transfected to over-express TLR2(Pue+oe-TLR2 group).The levels of TLR2 proteins were determined by western blotting and the levels of interferon-γ(IFN-γ)were determined by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the control group,there was no significant change in the proliferation of 3T3-L1 adipocytes in the puerarin treatment groups(P>0.05).Compared with the control group,the differentiation of 3T3-L1 adipocytes was significantly promoted in the puerarin treatment groups(P<0.05).Compared with the control group,the glucose consumption of IR 3T3-L1 adipocytes was significantly increased in the puerarin treatment groups(P<0.05).The expression of TLR2 and the secretion of IFN-γin IR 3T3-L1 adipocytes were decreased,and the levels of GLUT4 and PPARγwere increased in the puerarin treatment groups(P<0.05).The relative expression of TLR2 and the secretion of IFN-γin the Pue+oe-TLR2 group were significantly higher than those in the Pue+oe-NC group,and the relative expression of PPARγand GLUT4 was significantly lower than that in the Pue+oe-NC group(P<0.01).Conclusion:Pue-rarin can increase the glucose consumption of IR3T3-L1 adipocytes and alleviate IR.Its mechanism may be related to the inhibition of TLR2 expression and the reduction of IFN-γsecretion.

关 键 词：葛根素 TLR2 干扰素-Γ 3T3-L1脂肪细胞 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学]