短期大量摄入酒精对树鼩行为学及运动机能的影响  

Effect of short-term high alcohol intake on behavior and motor function of Tupaia belangeri chinensis

作　　者：郑思聪 罗秋妮 彭碧燕 肖林林 罗兰诗雨 杨逸萱 尹思威 欧阳轶强 施维 李习艺[3] ZHENG Sicong;LUO Qiuni;PENG Biyan;XIAO Linlin;LUO Lanshiyu;YANG Yixuan;YIN Siwei;OUYANG Yiqiang;SHI Wei;LI Xiyi(Laboratory Animal Center,Guangxi Medical University,Nanning 530021,China;School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China;Nutrition and Food Hygiene,School of Public Health,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学实验动物中心,南宁530021 [2]广西医科大学基础医学院,南宁530021 [3]广西医科大学公共卫生学院营养与食品安全系,南宁530021

出　　处：《广西医科大学学报》2024年第1期46-50,共5页Journal of Guangxi Medical University

基　　金：广西自然科学基金项目(No.2023GXNSFDA026069)。

摘　　要：目的:探讨短期大量摄入酒精对树鼩行为学及运动机能的影响,为后续神经科学研究奠定基础。方法:用不同体积的50%乙醇溶液构建树鼩酒精暴饮模型。将20只雄性滇西树鼩随机分为对照组、低剂量组、中剂量组与高剂量组,每组5只。采用旷场试验、步态分析试验综合评价树鼩饮酒前后的行为学基本特征,比较3个剂量组的运动总距离、平均速度、最高速度、穿越中央区次数以及步态线性度Δα、交替系数β、步长规律系数σ。结果:旷场试验显示,与对照组相比,中、高剂量组的运动总距离、平均速度、最高速度均显著降低(均P<0.01),3个剂量组树鼩穿越中央区次数均显著减少(均P<0.01),且高剂量组的变化最为明显。步态分析显示,与对照组相比,中、高剂量组树鼩的步态线性度Δα均显著升高(均P<0.01),3个剂量组的交替系数β、步长规律系数σ均显著升高(均P<0.01),且高剂量组升高最为明显。结论:树鼩短期大量摄入酒精对其身体产生明显的镇静作用,表现为探索欲望降低,步态紊乱,甚至共济失调。Objective:To investigate the effect of short-term high alcohol intake on the behavior and motor function of Tupaia belangeri chinensis(T.b.chinensis),so as to lay the foundation for further neuroscience research.Methods:An alcohol binge-drinking model was established on T.b.chinensis by giving 50%ethanol solutions of various volumes.Twenty male T.b.chinensis were randomly divided into control,low-dose,medium-dose,and high-dose groups,with 5 individuals per group.Open field test and gait analysis were used to comprehensively evaluate the basic behavioral characteristics of T.b.chinensis before and after drinking.The total movement distance,average speed,highest speed,number of crossings through the central area,gait linearityΔα,alternation coefficientβ,and step regularity coefficientσwere compared among the three dose groups.Results:The open field experiment showed that the total movement distance,the average speed and the highest speed of T.b.chinensis in the medium-and high-dose groups were significantly deceased in comparison of those in the control group(all P<0.01),while the number of crossings through the central area was significantly decreased in all three dose groups(all P<0.01).Among them,the changes were most obvious in the high-dose group.Gait analysis showed that compared with the control group,there was a significant increase of the gait linearityΔαof T.b.chinensis in the medium-and high-dose groups(all P<0.01),and of the alternate coefficientβand the step regularity coefficientσin all three dose groups(all P<0.01),with the high-dose group showing the most significant increase.Conclusion:Short-term high alcohol intake has a significant sedative effect on T.b.chinensis,manifested by a decreased desire for exploration,disordered gait and even ataxia.

关 键 词：树鼩 动物行为 酒精 旷场试验 步态分析 

分 类 号：R332[医药卫生—人体生理学]