PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能  

Combined detection of PAX1 methylation and p16/Ki-67 dual staining improves diagnostic performance for atypical squamous cells in cervical cancer

作　　者：王元培 刘雨晨 温静 孙怡 程晓冉 任芳[1] WANG Yuanpei;LIU Yuchen;WEN Jing;SUN Yi;CHENG Xiaoran;REN Fang(Department of Gynecology,First Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan Province,450052,China)

机构地区：[1]郑州大学第一附属医院妇科,郑州450052

出　　处：《陆军军医大学学报》2024年第4期391-395,共5页Journal of Army Medical University

基　　金：河南省科技发展计划优秀青年科学基金(222300420091)。

摘　　要：目的 比较配对盒家族基因1(paired boxed gene 1,PAXl)甲基化与p16/Ki-67双染单独及联合检测在薄层液基细胞学检查(thinprep cytologic test, TCT)为意义不明的非典型鳞状细胞(atypical squamous cells of undetermined significance, ASC-US)及低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion, LSIL)人群中的诊断效能。方法 选择郑州大学第一附属医院2021-2022年247例TCT结果为ASC-US和LSIL患者,以阴道镜下病理结果为金标准,敏感度、特异性、准确度与ROC曲线下面积(area under the subject curve, AUC)为评价指标,比较PAX1甲基化和p16/Ki-67双染单独及联合检测的检验效能。结果 PAX1甲基化与p16/Ki-67双染的阳性率随着阴道镜下病理结果的严重程度而增加,PAX1甲基化和p16/Ki-67联合检测在TCT为ASC-US人群中的敏感度为91.25%、特异度为97.72%、准确度为95.51%,优于甲基化、p16/Ki-67双染单独检测,差异有统计学意义(P<0.01)。结论 PAX1甲基化和p16/Ki-67双染联合检测可以提高TCT结果为ASC-US患者的诊断效能。Objective To compare paired boxed gene 1(PAX1)methylation and p16/Ki-67 double staining alone and in combination in thinprep cytologic test(TCT)for distinguishing atypical squamous cells of undetermined significance(ASC-US)and low-grade squamous intraepithelial lesion(LSIL)population.Methods A total of 247 patients with TCT results of ASC-US and LSIL admitted in our hospital from January 2021 to December 2022 were enrolled in this study.Detection efficacy of PAX1 methylation and p16/Ki-67 double staining alone and in combination was evaluated with colposcopic pathologic results as the gold standard and the sensitivity,specificity,accuracy and area under the curve(AUC)as evaluation indexes.Results The positive rates of PAX1 methylation and p16/Ki-67 double staining were increased with the severity of pathological findings.Combined detection of PAX1 methylation and p16/Ki-67 assay had a sensitivity of 91.25%,specificity of 97.72%and accuracy of 95.51%in the women with TCT of ASC-US,and these values were statistically better than those of PAX1 methylation and p16/Ki-67 double staining alone(P<0.01).Conclusion The combination of PAX1 methylation and p16/Ki-67 double-staining assay can further improve the diagnostic efficacy in patients with ASC-US on TCT results.

关 键 词：PAX1甲基化 p16/Ki-67双染 宫颈癌 薄层液基细胞学检查 

分 类 号：R446.8[医药卫生—诊断学] R730.43[医药卫生—临床医学] R737.33