Gibberellin promotes cambium reestablishment during secondary vascular tissue regeneration after girdling in an auxin-dependent manner in Populus  

作　　者：Yufei Zhang Lingyan Wang Yuexin Wu Donghui Wang Xin‐Qiang He 

机构地区：[1]State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China

出　　处：《Journal of Integrative Plant Biology》2024年第1期86-102,共17页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(32071726,32271825)to Xin-qiang He.

摘　　要：Secondary vascular tissue(SVT)development and regeneration are regulated by phytohormones.In this study,we used an in vitro SVT regeneration system to demonstrate that gibberellin(GA)treatment significantly promotes auxin-induced cambium reestablishment.Altering GA content by overexpressing or knocking down ent-kaurene synthase(KS)affected secondary growth and SVT regeneration in poplar.The poplar DELLA gene GIBBERELLIC ACID INSENSITIVE(PtoGAI)is expressed in a specific pattern during secondary growth and cambium regeneration after girdling.Overexpression of PtoGAI disrupted poplar growth and inhibited cambium regeneration,and the inhibition of cambium regeneration could be partially restored by GA application.Further analysis of the PtaDR5:GUS transgenic plants,the localization of PIN-FORMED 1(PIN1)and the expression of auxin-related genes found that an additional GA treatment could enhance the auxin response as well as the expression of PIN1,which mediates auxin transport during SVT regeneration.Taken together,these findings suggest that GA promotes cambium regeneration by stimulating auxin signal transduction.

关 键 词：auxin CAMBIUM GIBBERELLIN POPULUS secondary vascular tissue regeneration 

分 类 号：S792.11[农业科学—林木遗传育种]