基于UHPLC-Q-TOF-MS/MS技术的百蕊颗粒入血成分研究  

Analysis on blood-absorbed components of Bairui Granules based on UHPLC-Q-TOF-MS/MS technology

作　　者：周思倩 方艳[2] 谭金桃[2] 王雅静 吴娟 邵乐[1] 龙红萍 ZHOU Si-qian;FANG Yan;TAN Jin-tao;WANG Ya-jing;WU Juan;SHAO Le;LONG Hong-ping(The First Hospital of Hunan University of Chinese Medicine;Department of Pharmacy,Hunan Children’s Hospital,Changsha 410007,China;Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院 [2]湖南省儿童医院药学部,长沙410007 [3]湖南中医药大学,长沙410208

出　　处：《天然产物研究与开发》2024年第2期243-251,共9页Natural Product Research and Development

基　　金：湖南省卫生健康委科研项目(202113051694);湖南省教育厅优秀青年基金(21B0371)。

摘　　要：采用超高效液相色谱-四极杆飞行时间质谱(UHPLC-Q-TOF-MS/MS)技术辨析百蕊颗粒灌胃大鼠后的原型成分和代谢产物,并研究其主要体内代谢过程。大鼠口服给药百蕊颗粒后,采用UHPLC-Q-TOF-MS/MS技术采集血清样本数据,正、负离子模式下同时扫描,结合前期体外化学成分质谱数据以及提取各化学成分的精确质荷比等信息对百蕊颗粒在大鼠体内的原型成分和代谢产物进行辨识研究。结果在大鼠体内中共检测到20个原型成分和10个代谢产物,其中原型成分包括生物碱类化合物6个,黄酮类化合物4个,有机酸类3个等其他化合物,主要代谢途径包括还原反应和水解反应、硫酸化反应和葡萄糖醛酸结合反应。该研究为揭示百蕊颗粒药效物质基础奠定了基础,也为其中成药的开发与利用提供应用价值。Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS/MS)was used to identify the prototypical components and metabolites of Bairui Granules after gavage in rats,and to study the major in vivo metabolic processes.After oral administration of Bairui Granules to rats,blood sample data were collected by UHPLC-Q-TOF-MS/MS,scanned simultaneously in positive and negative ion mode,and combined with the preliminary in vitro chemical compounds data as well as extracting the precise mass-to-charge ratios of each chemical components to carry out the study on the identification of the prototypical components and metabolites of Bairui granules in rats.As a result,a total of twenty prototypical components and ten metabolites were detected in rats,of which the prototypical components included six alkaloids,four flavonoids,three organic acids,etc.The main metabolic process included reduction and hydrolysis reaction,sulphation reaction and glucuronide binding reaction.This study provided a foundation for understanding the pharmacologically active substances in Bairui Granules and offered valuable insights into the development and utilization of the formulated medications.

关 键 词：百蕊颗粒 UHPLC-Q-TOF-MS/MS 入血成分 代谢产物 代谢过程 

分 类 号：R917[医药卫生—药物分析学]