用于荧光显微技术的多波长激光耦合系统  

Multi-wavelength lasers coupling system used in fluorescent microscopy

作　　者：周旻超 王振亚[2] 方懿 罗刚银 张哲[2] 孙晓洁 ZHOU Minchao;WANG Zhenya;FANG Yi;LUO Gangyin;ZHANG Zhe;SUN Xiaojie(Academy for Engineering and Technology,Fudan University,Shanghai 200433,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215000,China)

机构地区：[1]复旦大学工程与应用技术研究院,上海200433 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215163

出　　处：《光学精密工程》2024年第3期324-332,共9页Optics and Precision Engineering

基　　金：中国科学院科研仪器设备研制项目(No.Y822141202)。

摘　　要：为了满足荧光显微镜技术对多波长单模耦合输出激光的需求,研究了400~680 nm内多波长激光耦合进单模光纤的技术,设计了三片式耦合透镜组很好地消除了不同波长耦合时的色差以提高耦合效率,同时考虑光纤耦合调试难度大的问题,设计了能快速简单完成耦合调节的耦合光纤部件结构以及耦合调试的方法,通过实验测试荧光成像常用的4种波段激光(405,488,561,638 nm),耦合效率均大于65%。实验结果达到了较高的光纤耦合水平,证明该多波长激光耦合器性能优异。同时,由于耦合器装调简单且成本低,本文的工作具有进一步商业化的价值,且为多波长单模耦合激光器国产化打下了坚实的基础。The rapid advancement of fluorescence microscopy technology has increased the need for better excitation light sources,particularly for multi⁃wavelength lasers coupled in single mode fiber.This article explores the technology of coupling multi⁃wavelength lasers into single mode fibers within the spectral range of 400-680 nm.To enhance the coupling efficiency of different wavelengths,a triplet lens is de⁃signed to effectively reduce chromatic aberration.Moreover,a fiber coupling component structure and a quick and easy coupling adjustment method was developed to address the challenges of fiber coupling align⁃ment.Fiber coupling experiments involving four commonly used laser wavelengths in fluorescence microsco⁃py(405,488,561,638 nm)have demonstrated a coupling efficiency of over 65%for all four wavelengths.These results validate the effectiveness of the multi⁃wavelength lasers coupler designed in this study.Further⁃more,due to its simple adjustment and low cost,the coupler holds significant commercial value and estab⁃lishes a solid foundation for the localization of multi⁃wavelength lasers coupled in single mode fiber.

关 键 词：荧光显微技术 单模激光耦合 多波长激光 光纤耦合 

分 类 号：TN248.4[电子电信—物理电子学]