胰岛素对昆虫病原线虫卡森斯氏线虫0657L生长发育及其共生细菌伯氏致病杆菌0657L的影响  

Effects of insulin on the growth and development of the entomopathetic nematode Steinernema kraussei 0657L and its symbiotic bacterium Xenorhabdus bovenii 0657L

作　　者：李茜童 陈红 王兴铎 钱秀娟 LI Xi-Tong;CHEN Hong;WANG Xing-Duo;QIAN Xiu-Juan(Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《昆虫学报》2024年第1期38-47,共10页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31960559);甘肃省科技计划项目(21JR7RA819);西藏自治区科技计划项目(XZ202301ZY0019N)。

摘　　要：【目的】为探讨胰岛素对昆虫病原线虫卡森斯氏线虫Steinernema kraussei 0657L生长发育及其共生细菌的影响。【方法】吸取1.5 mL 3000头/mL的卡森斯氏线虫0657L悬浮液侵染大蜡螟Galleria mellonella 3龄幼虫,25℃培养24 h后分离卡森斯氏线虫0657L的共生细菌伯氏致病杆菌Xenorhabdus bovenii 0657L;分别用1∶500胰岛素和1∶5000胰岛素处理伯氏致病杆菌0657L后饲喂卡森斯氏线虫0657L,借助体视显微镜观测伯氏致病杆菌0657L在胰岛素处理1~6 d时的菌落直径并计算生长速率,计算卡森斯氏线虫0657L寿命、存活率、每30 s头部摆动次数和每30 s吞咽次数,评价其平均寿命、运动能力及吞咽能力。【结果】与空白对照组相比,1∶5000胰岛素和1∶500胰岛素处理组伯氏致病杆菌0657L的菌落直径在对数生长期(处理2 d时)可分别增大77.2%和54.0%,生长速率可由空白对照组的68.0μm/d分别提高至120.4和104.7μm/d;卡森斯氏线虫0657L寿命分别延长27.3%和45.5%;卡森斯氏线虫0657L 3龄幼虫每30 s头部摆动次数分别提高56.3%和74.7%,3和4龄幼虫及成虫每30 s吞咽次数分别提高50.9%和65.5%,26.8%和35.2%及23.7%和21.1%。【结论】胰岛素可以显著促进卡森斯氏线虫0657L共生细菌伯氏致病杆菌0657L单菌落的生长和提高其生长速率,能够通过伯氏致病杆菌0657L间接延长卡森斯氏线虫0657L的寿命,提高昆虫病原线虫的运动和吞咽能力。本研究结果可以为延长昆虫病原线虫商品货架期提供科学依据,为昆虫病原线虫的高效生物防治提供理论基础。【Aim】To investigate the effects of insulin on the growth and development of the entomopathogenic nematode Steinernema kraussei 0657L and its symbiotic bacterium.【Methods】The 3rd instar larvae of Galleria mellonella were infected with 1.5 mL of 3000/mL S.kraussei 0657L suspension,the symbiotic bacterium Xenorhabdus bovenii 0657L was isolated from S.kraussei 0657L after 24 h culture at 25℃.S.kraussei 0657L was fed with X.bovenii 0657L treated with 1∶500 and 1∶5000 insulin,respectively,the colonial diameter and growth rate of X.bovenii 0657L were measured by stereomicroscope at 1-6 d after insulin treatment,the lifespan,survival rate,number of head swing per 30 s and number of swallows per 30 s of S.kraussei 0657L were calculated to evaluate its average lifespan,locomotion and swallowing ability.【Results】The colonial diameters of X.bovenii 0657L in 1∶5000 insulin and 1∶500 insulin treatment groups were increased by 77.2% and 54.0%,respectively,during the logarithmic growth period(at 2 d after treatment)as compared with that of the blank control group.The growth rates of X.bovenii 0657L in 1∶5000 insulin and 1∶500 insulin treatment groups were increased from 68.0μm/d of the blank control group to 120.4 and 104.7μm/d,respectively.The lifespans of S.kraussei 0657L in 1∶5000 insulin and 1∶500 insulin treatment groups were increased by 27.3% and 45.5%,respectively,as compared with that of the blank control group.The numbers of head swing per 30 s of the 3rd instar larvae of S.kraussei 0657L in 1∶5000 insulin and 1∶500 insulin treatment groups were increased by 56.3%and 74.7%,respectively,as compared with that of the blank control group.The numbers of swallows per 30 s of the 3rd and 4th instar larvae and adults of S.kraussei 0657L in 1∶5000 insulin and 1∶500 insulin treatment groups were increased by 50.9% and 65.5%,26.8% and 35.2%,23.7% and 21.1%,respectively,as compared with that of the blank control group.【Conclusion】Insulin can significantly promote the growth and increase

关 键 词：卡森斯氏线虫 伯氏致病杆菌 胰岛素 昆虫病原线虫 共生细菌 生长发育 寿命 运动 吞咽 

分 类 号：S476[农业科学—农业昆虫与害虫防治]