超高效液相色谱-三重四极杆质谱法测定化妆品中8种替丁类禁用药物  

Determination of 8 Prohibited Tidine Drugs in Cosmetics by Ultra High Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

作　　者：张秋炎 梁维维 罗辉泰 廖均涛 黄芳 吴惠勤 ZHANG Qiu-yan;LIANG Wei-wei;LUO Hui-tai;LIAO Jun-tao;HUANG Fang;WU Hui-qin(Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine,Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou 510070,China)

机构地区：[1]广东省科学院测试分析研究所(中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省中药质量安全工程技术研究中心,广东广州510070

出　　处：《分析测试学报》2024年第2期285-291,共7页Journal of Instrumental Analysis

基　　金：广东省科学院打造综合产业技术创新中心行动资金项目“高端医疗健康与高效生物转化关键技术研究及应用”(2022GDASZH-2022010110)。

摘　　要：建立了同时测定5种不同基质类型化妆品中8种替丁类药物的超高效液相色谱-三重四极杆质谱法(UHPLC-MS/MS)。不同基质类型化妆品样品经水-乙腈(2∶8,体积比)涡旋混匀、超声提取后过滤上机分析。选用YMC-Triart C_(18)色谱柱(100 mm×2.1 mm,3.0μm),以0.2%甲酸溶液-乙腈为流动相进行梯度洗脱分离,质谱分析采用电喷雾正离子模式和多反应监测模式。实验表明,8种替丁类药物在各自质量浓度范围内线性关系良好,相关系数(r^(2))为0.9975~0.9986,检出限为0.06~0.3μg/L,定量下限为0.2~1.0μg/L。在3个不同加标水平下,水剂类、膏霜类、乳液类、凝胶类和粉剂类样品的平均回收率分别为80.7%~106%、90.1%~102%、82.6%~101%、82.0%~110%和86.6%~101%;相对标准偏差为0.70%~7.9%。该法前处理过程简单高效,易操作,灵敏度高,适用于上述5种类型化妆品中8种替丁类药物的同时快速定性定量分析。A new method for the simultaneous and rapid determination of 8 tidine drugs in 5 different matrix types of cosmetics by ultra high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS)was established.The different matrix cosmetic samples were dipersed with water-acetonitrile(2∶8,volume ratio)and ultrasonic extraction,and the filtrate was analyzed by UHPLC-MS/MS.A YMC-Triart C_(18) column(100 mm×2.1 mm,3.0μm)was used as separation chromatographic column,with 0.2%formic acid aqueous solution and acetonitrile as mobile phases under gradient elution.Eight tidines drugs were analyzed in the positive mode using multiple reaction monitoring mode and electrospray ionization mass spectrometry.The results showed that the correla-tion coefficients for linear calibration curves were ranged of 0.9975-0.9986 in the corresponding concentration range.The limits of detection(LODs)of 8 tidine drugs were in the range of 0.06-0.3μg/L,and the limits of quantitation(LOQs)were in the range of 0.2-1.0μg/L.The average recover-ies of 8 tidine drugs from water aqua,cream,lotion,gel and powder samples at three different spiked levels ranged of 80.7%-106%,90.1%-102%,82.6%-101%,82.0%-110%and 86.6%-101%,re-spectively,with relative standard deviations ranged of 0.70%-7.9%.The established method is sim-ple,effective,easy to operate,high sensitive,and accurate,and is suitable for the determination of 8 tidine drugs in 5 different matrixes of cosmetics(including water aqua,cream,lotion,gel and powder samples).

关 键 词：超高效液相色谱-三重四极杆质谱法 化妆品 抗组胺 替丁类药物 

分 类 号：O657.7[理学—分析化学] TQ658[理学—化学]