腺病毒介导的肝细胞生长因子/血管内皮生长因子165对大鼠超比例随意皮瓣成活的影响及机制研究  

Experimental Study on the Influence of Adenovirus-mediated Hepatocyte Growth Factor/vascular Endothelial Growth Factor 165 on the Survival and Mechanism of Hyper-proportion Random Flap in Rats

作　　者：赵毅 闫洪伟 王玉琦 陈丽娟 洪城 田诗政 ZHAO Yi;YAN Hongwei;WANG Yuqi;CHEN Lijuan;HONG Cheng;TIAN Shizheng(Jinzhou Medical University,Jinzhou 121000,Liaoning,China;Department of Burn Plastic Surgery,Shiyan People's Hospital/Affiliated People's Hospital of Hubei University of Medicine,Shiyan 442000,Hubei,China)

机构地区：[1]锦州医科大学,辽宁锦州121000 [2]湖北医药学院附属人民医院烧伤整形科,湖北十堰442000

出　　处：《中国美容医学》2024年第2期60-63,97,共5页Chinese Journal of Aesthetic Medicine

基　　金：湖北省十堰市科技局指导项目(编号:21Y61)。

摘　　要：目的:观察术前注射腺病毒介导的肝细胞生长因子/血管内皮生长因子165(Ad-HGF/VEGF165)对大鼠超比例随意皮瓣成活的影响和其机制的初步研究。方法:大鼠背部设计超比例随意皮瓣模型,将其随机分为Ad-HGF/VEGF165组、AdVEGF165组和对照组。术前7 d,Ad-HGF/VEGF165组和Ad-VEGF165组分别于大鼠皮瓣及创缘内多点注射Ad-HGF/VEGF165及AdVEGF165,对照组注射等容量0.9%氯化钠注射液。分别于术后7 d、14 d观测皮瓣成活面积,切取皮瓣组织标本HE染色、免疫组化观察微血管密度及组织间VEGF阳性率等指标,WesternBlot检测目的蛋白表达情况。结果:术后7 d、14 d,Ad-HGF/VEGF165组、Ad-VEGF165组皮瓣存活面积、组织间微血管数目、VEGF及细胞外调节蛋白激酶(ERK1/2)蛋白表达情况均高于对照组(P<0.001),且Ad-HGF/VEGF165组明显高于Ad-VEGF165组(P<0.05)。结论:Ad-HGF/VEGF165具有协同作用,能显著促进VEGF和ERK1/2信号因子表达,短期内促进血管网重建,改善皮瓣血液灌注,提高大鼠超比例随意皮瓣成活面积。Objective To observe the effect of adenovirus-mediated hepatocyte growth factor/vascular endothelial growth factor 165(Ad-HGF/VEGF165)injection before flap operation on the survival and Mechanism of Hyper-proportion random flap in rats.Methods The model of Hyper-proportion random flap was designed on the back of rats and randomly divided into Ad-HGF/VEGF165 group,Ad-HGF/VEGF165 group and control group.Seven days before operation,Ad-HGF/VEGF165 group and Ad-HGF/VEGF165 group were separate multipoint injection Ad-HGF/VEGF165 and Ad-VEGF165 into the rat skin flap at multiple points in the experimental group,while 0.9%sodium chloride injection was injected in the control group.The survival area of the flap was observed on the 7 day and 14 day after operation,and the tissue samples of the flap were obtained for HE staining,the microvessel density and VEGF expression were observed by immunohistochemistry,and Western blot for detection of protein of target protein.Results On the 7 day and 14 day after operation,skin flap survival area,the number of microvessels,VEGF and the expression of extracellular regulated protein kinase(ERK1/2)protein in the Ad-HGF/VEGF165 group and Ad-HGF/VEGF165 group were higher than those in the control group(P<0.001),and all indexes were significantly higher in the Ad-HGF/VEGF165 group than in the Ad-VEGF165 group(P<0.05).Conclusion Ad-HGF/VEGF165 has a synergistic effect,which can significantly promote the expression of VEGF and ERK1/2 signal factors,increase the survival area of Hyper-proportion random flap,promote angiogenesis and improve the blood circulation of the flap.

关 键 词：超比例随意皮瓣 基因治疗 腺病毒 血管新生 肝细胞生长因子 血管内皮生长因子 

分 类 号：R622[医药卫生—整形外科]