COPD细胞微环境体外模型建立的思路与方法  

Ideas and methods of in vitro model establishmentfor cell microenvironment in COPD

作　　者：秦燕勤 刘含 李建生[1,3] QIN Yan-qin;LIU Han;LI Jian-sheng(Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine,Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry of P.R.China,Zhengzhou 450046,China;Academy of Chinese Medical Sciences,Henan University of Chinese Medicine,Zhengzhou 450046,China;the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450003,China)

机构地区：[1]河南中医药大学,河南省中医药防治呼吸病重点实验室,呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046 [2]河南中医药大学中医药科学院,河南郑州450046 [3]河南中医药大学第一附属医院呼吸科,河南郑州450003

出　　处：《中国药理学通报》2024年第1期6-12,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82104662);河南省重点研发与推广项目(科技攻关)(No 222102310141);河南省高等学校重点科研项目(No 22A360007);河南省博士后科研项目(No 202101046)。

摘　　要：慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)是危害公众健康的重大慢性疾病。COPD病理机制复杂,细胞微环境改变是其病理生理的重要组成部分。细胞培养技术是研究COPD病理机制及药效药理评价的重要工具。该文介绍了肺内细胞微环境的基本构成,COPD病理进程中细胞微环境变化,总结了细胞微环境体外模型在COPD机制研究中的应用,提出COPD细胞微环境体外模型建立的思路与方法。Chronic obstructive pulmonary disease(COPD)is major chronic disease threatening public health with complex pathological mechanisms.The change of the cell microenvironment of the lung is an important part of the pathophysiology of COPD.Cell culture technology is an important method to investigate the pathological mechanism of COPD and evaluate the pharmacological effect of medicine.Here we introduce the composition of the cell microenvironment of the lung,the change of the cell microenvironment in the pathological process of COPD,and summarize the application of in vitro model mimics cell microenvironment of COPD in the study of mechanism.In addition,we aim to put forward the ideas of the in vitro model establishment of cell microenvironment of COPD.

关 键 词：COPD 细胞微环境 体外模型 细胞共培养 气-液面培养 气血屏障 肺类器官 

分 类 号：R322.35[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学] R563.9